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Development and Validation of a Method for Assay of the Original Antituberculosis Agent Thiozonide in Plasma for Pharmacokinetic Studies

  • STRUCTURE OF CHEMICAL COMPOUNDS, METHODS OF ANALYSIS AND PROCESS CONTROL
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Pharmaceutical Chemistry Journal Aims and scope

A method for assaying the antituberculosis drug thiozonide in plasma was developed and validated. Sample preparation consisted of precipitation of proteins with acetonitrile. Assay was by HPLC with a mass-selective detector. The method developed here was validated in terms of the following validation properties: selectivity, linearity, accuracy, precision, quantitative detection limit, sample transfer, and solution stability. The analytical range of the method was 1 – 1000 ng/ml thiozonide in plasma. This analytical range allows this method to be used for further studies of the pharmacokinetics of the innovative drug thiozonide.

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Authors and Affiliations

  1. First Moscow I. M. Sechenov State Medical University, 8 Trubetskaya Street, 119991, Moscow, Russia

    G. V. Ramenskaya, I. E. Shokhin, L. A. Men’shikova, A. Yu. Savchenko, V. V. Smirnov & E. A. Egorenkov

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  1. G. V. Ramenskaya
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  2. I. E. Shokhin
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  3. L. A. Men’shikova
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  4. A. Yu. Savchenko
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  5. V. V. Smirnov
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  6. E. A. Egorenkov
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Corresponding author

Correspondence to G. V. Ramenskaya.

Additional information

Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 49, No. 3, pp. 46 – 10, March, 2015.

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Ramenskaya, G.V., Shokhin, I.E., Men’shikova, L.A. et al. Development and Validation of a Method for Assay of the Original Antituberculosis Agent Thiozonide in Plasma for Pharmacokinetic Studies. Pharm Chem J 49, 199–202 (2015). https://doi.org/10.1007/s11094-015-1254-4

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  • DOI: https://doi.org/10.1007/s11094-015-1254-4

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