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Light scattering properties in spatial planes for label free cells with different internal structures

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Abstract

Label free cells properties of 2D scattering intensity spectra and 3D scattering images in different spatial fields and planes are studied according to three dimensional finite-difference time-domain simulation results. The correlation of intensity spectra and scattering images in different spatial regions are discussed with different cells nucleus size, nucleus off-center situations, small organelles existence and distribution. Compared the scattering information, the most sensitive spatial regions to cells structures changing are figured out which can be selected to improve label free cells distinguishing accuracy. The research results would be very important to fully discover cells spatial scattering features and provide more reliable sorting criterions for label free cells detection technologies.

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Acknowledgments

This study was funded by China Postdoctoral Science Foundation (Grant No. 2012M521759), Higher School Specialized Research Fund for the Doctoral Program Funding Issue (Grant No. 2011021120032), the Fundamental Research Funds for the Central Universities (Grant Nos. 08142010 and 2010jdhz23), National Natural Science Foundation of China Major Research Program on Nano-manufacturing (Grant No. 91323303), Shaanxi Province Natural Science Foundation Research Project(Grant No. 2014JQ8334).

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Authors and Affiliations

  1. State Key Laboratory for Manufacturing System Engineering, School of Mechanical Engineering, Xi’an Jiaotong University, Xi’an , 710049, Shaanxi, China

    Lu Zhang, Yuwei Qin, Kai-Xing Li, Xin Zhao, Yun-Fei Xing, Hong Zhao & Zhuangde Jiang

  2. School of Physics and Electrical Engineering, Weinan Normal University, Weinan , 714000, China

    Yuwei Qin

  3. Department of Laboratory Medicine, The First Affiliated Hospital, Xi’an Jiaotong University, Xi’an , 710049, Shaanxi, China

    Wei Chen & Li Yuan

  4. Xijing Hospital, The Fourth Military Medical University, Xi’an , 700032, Shaanxi, China

    Nian-Ling Yao

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  1. Lu Zhang
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  2. Yuwei Qin
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  3. Kai-Xing Li
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  4. Xin Zhao
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  5. Yun-Fei Xing
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  6. Hong Zhao
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  8. Wei Chen
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  9. Nian-Ling Yao
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  10. Li Yuan
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Correspondence to Lu Zhang.

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Zhang, L., Qin, Y., Li, KX. et al. Light scattering properties in spatial planes for label free cells with different internal structures. Opt Quant Electron 47, 1005–1025 (2015). https://doi.org/10.1007/s11082-014-9957-4

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  • DOI: https://doi.org/10.1007/s11082-014-9957-4

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