Abstract
Peripheral nerve injury and regeneration is a complex biological process jointly mediated by numerous factors. Cyclic adenosine monophosphate (cAMP) modifies the cellular behaviors of neurons and Schwann cells, and thus may contribute to peripheral nerve regeneration. Despite the importance of cAMP, the temporal and spatial expressions of genes involved in cAMP-related signaling pathways during peripheral nerve regeneration remain unclear. In the current study, by using rat sciatic nerve crush model, we analyzed previously obtained RNA deep sequencing data, explored the significance of cAMP-mediated signaling pathway and protein kinase A (PKA) signaling pathway after peripheral nerve injury, and examined the expression patterns of genes involved in these cAMP-related signaling pathways. Our results, from the genetic aspect, emphasized the critical involvement of cAMP-related signaling pathways, identified the dynamic changes of some key signaling cascades, and may help the discovery of potential therapeutic targets for peripheral nerve repair and regeneration.
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Acknowledgements
The authors are grateful to Professor Jie Liu at Nantong University for his help in the preparation of the manuscript. This work was supported by the Natural Science Foundation of Jiangsu Province, China (BK20150409); and Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
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SY conceived and designed the experiments. JY, SW, CW, and SY performed the experiments. JY and SY analyzed the data. SY Contributed reagents/materials/analysis tools. SY wrote the manuscript.
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11064_2017_2409_MOESM1_ESM.xlsx
Table S1. Differentially expressed genes in cAMP-mediated signaling pathway. The expression levels of genes in cAMP-mediated signaling pathway at 1, 4, 7, and 14 days after sciatic nerve crush were compared with 0 day and presented as log2Ratio (XLSX 35 KB)
11064_2017_2409_MOESM2_ESM.xlsx
Table S2. Differentially expressed genes in PKA signaling pathway. The expression levels of genes in PKA signaling pathway at 1, 4, 7, and 14 days after sciatic nerve crush were compared with 0 day and presented as log2Ratio (XLSX 48 KB)
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Yu, J., Wang, S., Wu, C. et al. Deep Sequencing Reveals the Significant Involvement of cAMP-Related Signaling Pathways Following Sciatic Nerve Crush. Neurochem Res 42, 3603–3611 (2017). https://doi.org/10.1007/s11064-017-2409-3
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DOI: https://doi.org/10.1007/s11064-017-2409-3