Abstract
Experimental autoimmune encephalomyelitis (EAE) is commonly induced with myelin oligodendrocyte glycoprotein (MOG)35–55; occasionally, EAE is not well induced despite MOG35–55 immunization. To confirm that EAE induction varies with difference in MOG35–55 properties, we compared three MOG35–55 from different commercial sources, which are MOG-A, MOG-B, and MOG-C. The peptides induced EAE disease with 100, 40, and 20 % incidence, respectively. Compared with others, MOG-A showed higher peptide purity (99.2 %) and content (92.2 %) and presented a sheet shape with additional sodium and chloride chemical elements. In MOG-A-treated group, MMP-9 activity and IL-6 levels were considerably higher than the other groups in CNS tissues, and significantly increased VCAM-1, IFN-γ, and decreased IL-4 were also shown compared to MOG-B- and/or MOG-C-treated group. In conclusion, the immunological and toxicological changes by the difference in MOG35–55 properties modulate EAE induction, and MOG35–55 which affects MMP-9 activity and IL-6 levels may be the most effective EAE-inducing antigen. This study can be potentially applied by researchers using MOG35-55 peptide and manufacturers for MOG35-55 synthesis.
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This work was supported by Korea Institute of Science and Technology (2V03600).
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Seo, JE., Hasan, M., Han, JS. et al. Dependency of Experimental Autoimmune Encephalomyelitis Induction on MOG35–55 Properties Modulating Matrix Metalloproteinase-9 and Interleukin-6. Neurochem Res 41, 666–676 (2016). https://doi.org/10.1007/s11064-015-1732-9
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DOI: https://doi.org/10.1007/s11064-015-1732-9