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Piperine Enhances the Protective Effect of Curcumin Against 3-NP Induced Neurotoxicity: Possible Neurotransmitters Modulation Mechanism

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Abstract

3-Nitropropionic acid (3-NP) is a fungal toxin well established model used for inducing symptoms of Huntington’s disease. Curcumin a natural polyphenol has been reported to possess neuroprotective activity by decreasing oxidative stress. The aim of present study was to investigate neuroprotective effect of curcumin with piperine (bioavailability enhancer) against 3-NP induced neurotoxicity in rats. Administration of 3-NP (10 mg/kg for 21 days) showed loss in body weight, declined motor function and changes in biochemical (LPO, nitrite and glutathione level), neuroinflammatory (TNF-α and IL-1β level) and neurochemical (DA, NE, 5-HT, DOPAC, 5-HIAA and HVA). Chronic treatment with curcumin (25 and 50 mg/kg) and curcumin (25 mg/kg) with piperine (2.5 mg/kg) once daily for 21 days prior to 3-NP administration. All the behavioral parameters were studied at 1st, 7th, 14th, and 21st day. On 22nd day all the animals was scarified and striatum was separated. Curcumin alone and combination (25 mg/kg) with piperine (2.5 mg/kg) showed beneficial effect against 3-NP induced motor deficit, biochemical and neurochemical abnormalities in rats. Piperine (2.5 mg/kg) with curcumin (25 mg/kg) significantly enhances its protective effect as compared with curcumin alone treated group. The results of the present study indicate that protective effect of curcumin potentiated in the presence of piperine (bioavailability enhancer) against 3-NP–induced behavioral and molecular alteration.

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Abbreviations

3-NP:

3-Nitropropionic acid

HD:

Huntington’s disease

LPO:

Lipid peroxidation

IL:

Interlukin

ROS:

Reactive oxygen species

TNF-α:

Tumour necrosis factor-alpha

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Acknowledgments

Authors are thankful to Science and Engineering Board (SERB), Department of Science and Technology, Govt. of India, New Delhi for providing financial assistance under Fast Track Scheme (DST-SERB-FTYS) to Dr. Puneet Kumar.

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Authors and Affiliations

  1. Department of Pharmacology, I.S.F College of Pharmacy, Ferozepur Road, Ghal Kalan, Moga, 142001, Punjab, India

    Shamsher Singh, Sumit Jamwal & Puneet Kumar

  2. Research Scholar, Punjab Technical University, Jalandhar, India

    Shamsher Singh & Sumit Jamwal

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  1. Shamsher Singh
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  2. Sumit Jamwal
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Correspondence to Puneet Kumar.

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Singh, S., Jamwal, S. & Kumar, P. Piperine Enhances the Protective Effect of Curcumin Against 3-NP Induced Neurotoxicity: Possible Neurotransmitters Modulation Mechanism. Neurochem Res 40, 1758–1766 (2015). https://doi.org/10.1007/s11064-015-1658-2

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