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Protocatechuic Acid Inhibits Inflammatory Responses in LPS-Stimulated BV2 Microglia via NF-κB and MAPKs Signaling Pathways

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Abstract

Protocatechuic acid (PA), a major metabolite of anthocyanins, has been reported to possess antioxidant and anti-inflammatory activities. However, the effects of PA on LPS-induced inflammatory responses in microglia have not been reported. The aim of this study was to investigate the anti-inflammatory effects and molecular mechanisms of PA on LPS-stimulated BV2 microglia. The production of inflammatory mediators TNF-α, IL-6, IL-1β, and PGE2 were detected by ELISA. TLR4, NF-κB and MAPKs activation were detected by western blotting. Our results demonstrated that PA dose-dependently inhibited LPS-induced TNF-α, IL-6, IL-1β, and PGE2 production. In addition, PA suppressed LPS-induced TLR4 expression, NF-κB and MAPKs activation, which resulted in the inhibition of inflammatory mediators. In conclusion, these results suggested that PA exhibited anti-inflammatory effects on LPS-stimulated BV2 microglia and the mechanisms were involved in the inhibition of TLR4-mediated NF-κB and MAPKs signaling pathways.

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Acknowledgments

This study was supported by Grants from the National Key disciplines Fund of the Ministry of Health of the People’s Republic of China, the Foundation of Tianjin Science and Technology Committee (12ZCDZSY17700, 14JCZDJC35600), National Key Technology Support Program 2014BAI04B02.

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All authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory, Tianjin Huanhu Hospital, Tianjin, 300060, China

    Huan-yu Wang, Hong Wang, Jin-huan Wang, Qiong Wang, Quan-feng Ma & Yi-yang Chen

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  1. Huan-yu Wang
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  2. Hong Wang
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  3. Jin-huan Wang
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Correspondence to Hong Wang.

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Wang, Hy., Wang, H., Wang, Jh. et al. Protocatechuic Acid Inhibits Inflammatory Responses in LPS-Stimulated BV2 Microglia via NF-κB and MAPKs Signaling Pathways. Neurochem Res 40, 1655–1660 (2015). https://doi.org/10.1007/s11064-015-1646-6

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  • DOI: https://doi.org/10.1007/s11064-015-1646-6

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