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Monocular Deprivation Delays the Dynamic Changes of Phosphorylated Synapsin Ia/b at Site-1 in Contralateral Visual Cortex of Juvenile Mice

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Abstract

Synapsins as a family of presynaptic terminal phosphoprotein participates in neuronal development, but their role in the synaptic plasticity of visual cortex is unclear. In this study, the impact of monocular deprivation (MD) on dynamic changes of isoform-specific protein expression and site 1 phosphorylation of synapsins in visual cortex of the postnatal mice were observed by using the technique of Western blot analysis. The results showed that the total (T-) protein levels of synapsins including the isoform of Ia/b, IIa/b and IIIa were about 21–26 % of adult level in visual cortex of mice at postnatal 7 days (P7), and then the T-synapsin Ia/b and IIb could quickly reach adult level at P35. However, the T-synapsin IIa and IIIa increased more slowly (71–74 % at P35), and then kept increasing in the visual cortex of mice at P60. Unlike to the changes of T-synapsins, the level of phosphorylated (P-) synapsin Ia/b (not IIa/b and IIIa) at site 1 increased with development to the highest level at P21, and then decreased rapidly to a low level in visual cortex of mice at P35–60. In addition, we found that the levels of P-synapsin Ia/b increased significantly in left visual cortex of P28 and P35 (not P21 and P42) mice with 1-week MD of right eye; and no significant changes of T-synapsins were observed in both left and right sides of visual cortex in P21–42 mice with MD treatment. These results suggested that the isoform-specific protein expression and site-1 phosphorylation of synapsins might play a different role in the synaptic plasticity of visual cortex, and MD delays the dynamic changes of phosphorylated synapsin Ia/b at site-1 in contralateral visual cortex of juvenile mice.

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Acknowledgments

This work was supported by grants from the Seed Grant of International Alliance of Translational Neuroscience (PXM2014-014226-000006), National Natural Science Foundation of China (Grant Nos. 31171147 and 31471142), Beijing Natural Science Foundation (Grant Nos. 7141001, 7132057), and Excellent Talent Training Aid from the Organization Department of Beijing Government (2010D003034000007).

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Authors and Affiliations

  1. Beijing Ophthalmology and Visual Science Key Laboratory, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China

    Tao Fu, Qing Su & Ping Xi

  2. Department of Neurobiology and Beijing Institute for Brain Disorders, Capital Medical University, #10 You An Men Wai Xi Tou Tiao, Beijing, 100069, China

    Song Han & Junfa Li

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  1. Tao Fu
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  2. Qing Su
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Correspondence to Tao Fu or Junfa Li.

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Fu, T., Su, Q., Xi, P. et al. Monocular Deprivation Delays the Dynamic Changes of Phosphorylated Synapsin Ia/b at Site-1 in Contralateral Visual Cortex of Juvenile Mice. Neurochem Res 40, 524–530 (2015). https://doi.org/10.1007/s11064-014-1492-y

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  • DOI: https://doi.org/10.1007/s11064-014-1492-y

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