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Uptake of Fluorescent Iron Oxide Nanoparticles by Oligodendroglial OLN-93 Cells

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Abstract

To investigate the cellular accumulation and intracellular localization of dimercaptosuccinate-coated iron oxide nanoparticles (D-IONPs) in oligodendroglial cells, we have synthesized IONPs that contain the fluorescent dye BODIPY (BP) in their coat (BP-D-IONPs) and have investigated the potential effects of the absence or presence of this dye on the particle uptake by oligodendroglial OLN-93 cells. Fluorescent BP-D-IONPs and non-fluorescent D-IONPs had similar hydrodynamic diameters and ζ-potentials of around 60 nm and −58 mV, respectively, and showed identical colloidal stability in physiological media with increasing particle size and positivation of the ζ-potential in presence of serum. After exposure of oligodendroglial OLN-93 cells to BP-D-IONPs or D-IONPs in the absence of serum, the specific cellular iron content increased strongly to around 1,800 nmol/mg. This strong iron accumulation was lowered for both types of IONPs by around 50 % on exposure of the cells at 4 °C and by around 90 % on incubation in presence of 10 % serum. The accumulation of both D-IONPs and BP-D-IONPs in the absence of serum was not affected by endocytosis inhibitors, whereas in the presence of serum inhibitors of clathrin-dependent endocytosis lowered the particle accumulation by around 50 %. These data demonstrate that oligodendroglial cells efficiently accumulate IONPs by an endocytotic process which is strongly affected by the temperature and the presence of serum and that BP-D-IONPs are a reliable tool to monitor by fluorescence microscopy the uptake and cellular fate of D-IONPs.

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Acknowledgments

The authors like to thank Christiane Richter-Landsberg (University of Oldenburg) for kindly supplying us with OLN-93 cells and Michaela C. Hohnholt (University of Bremen) for critically reading the manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Center for Biomolecular Interactions Bremen, University of Bremen, PO. Box 330440, 28334, Bremen, Germany

    Charlotte Petters, Felix Bulcke & Ralf Dringen

  2. Center for Environmental Research and Sustainable Technology, Leobener Strasse, 28359, Bremen, Germany

    Charlotte Petters, Felix Bulcke & Ralf Dringen

  3. Fraunhofer Institute for Manufacturing Technology and Advanced Materials, Wiener Strasse 12, 28359, Bremen, Germany

    Karsten Thiel

  4. Alfred-Wegener-Institut Helmholtz-Zentrum für Polar und Meeresforschung, Am Handelshafen 12, 27570, Bremerhaven, Germany

    Ulf Bickmeyer

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  1. Charlotte Petters
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  2. Felix Bulcke
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Correspondence to Ralf Dringen.

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Petters, C., Bulcke, F., Thiel, K. et al. Uptake of Fluorescent Iron Oxide Nanoparticles by Oligodendroglial OLN-93 Cells. Neurochem Res 39, 372–383 (2014). https://doi.org/10.1007/s11064-013-1234-6

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  • DOI: https://doi.org/10.1007/s11064-013-1234-6

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