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Capparis ovata Modulates Brain Oxidative Toxicity and Epileptic Seizures in Pentylentetrazol-Induced Epileptic Rats

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Abstract

It has been widely suggested that oxidative stress products play an important role in the pathophysiology of epilepsy. Capparis ovata (C. ovata) may useful treatment of epilepsy because it contains antioxidant flavonoids. The current study was designed to determine the effects of C. ovata on lipid peroxidation, antioxidant levels and electroencephalography (EEG) records in pentylentetrazol (PTZ)-induced epileptic rats. Thirty-two rats were randomly divided into four groups. First group was used as control although second group was PTZ group. Oral 100 and 200 mg/kg C. ovata were given to rats constituting the third and fourth groups for 7 days before PTZ administration. Second, third and forth groups received 60 mg/kg PTZ for induction of epilepsy. Three hours after administration of PTZ, EEG records, brain cortex and blood samples were taken all groups. The lipid peroxidation levels of the brain cortex, number of spikes and epileptiform discharges of EEG were higher in PTZ group than in control and C. ovata group whereas they were decreased by C. ovata administration. Vitamin A, vitamin C, vitamin E and β-carotene concentrations of brain cortex and latency to first spike of EEG were decreased by the PTZ administration although the brain cortex and plasma vitamin concentrations, and brain cortex and erythrocyte glutathione and glutathione peroxidase values were increased in PTZ + 100 and PTZ + 200 mg C. ovata groups. In conclusion, C. ovata administration caused protection against the PTZ-induced brain oxidative toxicity by inhibiting free radical and epileptic seizures, and supporting antioxidant redox system.

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Abbreviations

GSH:

Glutathione

GSH-Px:

Glutathione peroxidase

MDA:

Malondialdehyde

NO:

Nitric oxide

PTZ:

Pentylentetrazol

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

EEG:

Electroencephalography

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Acknowledgments

M.N. formulated the present hypothesis and was responsible for writing the report. Ö.Ç., M.B.A., M.İ.Y. and E.B. were responsible analyses the data. The study was partially supported as graduate student project by TUBITAK, Ankara, Turkey.

Conflict of interest

None.

Author information

Authors and Affiliations

  1. Neuroscience Research Center, Medical Faculty, Suleyman Demirel University, Tıp Fakultesi Dekanlik Binasi, 32260, Isparta, Turkey

    Mustafa Nazıroğlu & Ömer Çelik

  2. Department of Biophysics, Medical Faculty, Suleyman Demirel University, Isparta, Turkey

    Mustafa Nazıroğlu & Ömer Çelik

  3. Medical Faculty, Suleyman Demirel University, Isparta, Turkey

    Mehmet Berk Akay, Muhammed İkbal Yıldırım & Erdinç Balcı

  4. Department of Neurology, Medical Faculty, Suleyman Demirel University, Isparta, Turkey

    Vedat Ali Yürekli

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  1. Mustafa Nazıroğlu
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  2. Mehmet Berk Akay
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  3. Ömer Çelik
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  4. Muhammed İkbal Yıldırım
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Correspondence to Mustafa Nazıroğlu.

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Nazıroğlu, M., Akay, M.B., Çelik, Ö. et al. Capparis ovata Modulates Brain Oxidative Toxicity and Epileptic Seizures in Pentylentetrazol-Induced Epileptic Rats. Neurochem Res 38, 780–788 (2013). https://doi.org/10.1007/s11064-013-0978-3

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  • DOI: https://doi.org/10.1007/s11064-013-0978-3

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