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Comparison of the Immunoreactivity of Trx2/Prx3 Redox System in the Hippocampal CA1 Region Between the Young and Adult Gerbil Induced by Transient Cerebral Ischemia

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Abstract

In the present study, we compared the immunoreactivities and levels of Trx/prx redox system, thioredoxin 2 (Trx2), thioredoxin reductase 2 (TrxR2) and peroxiredoxin 3 (Prx3), as well as neuronal death in the hippocampal CA1 region between the adult and young gerbil after 5 min of transient cerebral ischemia. At 4 days post-ischemia, pyramidal neurons (about 90%) in the adult stratum pyramidale of the CA1 region showed “delayed neuronal death (DND)”; however, at this time point, few pyramidal neurons showed DND in the young stratum pyramidale. At 7 days post-ischemia, about 56% of pyramidal neurons showed DND in the young stratum pyramidale. The immunoreactivities of all the antioxidants in the young sham-group were similar to those in the adult sham-group. At 4 days post-ischemia, the immunoreactivity of TrxR2, not Trx2 and Prx3 in the adult ischemia-group was dramatically decreased in CA1 pyramidal neurons. At this time point, the immunoreactivities of all the antioxidants in the young ischemia-group were apparently increased compared to the adult ischemia-group. From 7 days pots-ischemia, non-pyramidal cells showed the immunoreactivities of all the antioxidants in the ischemic CA1 region; however, in the young ischemia-groups, the immunoreactivities were much lower than those in the adult ischemia-groups. In brief, our results showed that the immunoreactivities of Trx2, TrxR2 and Prx3 were dramatically increased in CA1 pyramidal neurons of the young ischemia-groups at 4 days post-ischemia compared to those in the adult ischemia-groups induced by transient cerebral ischemia.

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Acknowledgments

The authors would like to thank Mr. Seung Uk Lee for his technical help in this study. This work was supported by Rural Development Administration of Agenda project (PJ008261), Korea, and by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2011-0007307).

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Authors and Affiliations

  1. Department of Neurobiology, Institute of Medical Sciences, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea

    Bing Chun Yan, Joon Ha Park & Moo-Ho Won

  2. Laboratory of Neuroscience, Department of Physical Therapy, College of Rehabilitation Science, Daegu University, Gyeongsan, 712-714, South Korea

    Ji Hyeon Ahn

  3. Department of Emergency Medicine, Seoul Hospital, College of Medicine, Sooncheonhyang University, Seoul, 140-743, South Korea

    Young Joo Lee

  4. Department of Emergency Medicine, College of Medicine, Chuncheon Sacred Heart Hospital, Hallym University, Chuncheon, 200-702, South Korea

    Tae Hun Lee

  5. Department of Anatomy and Physiology, College of Pharmacy, Dankook University, Cheonan, 330-714, South Korea

    Choong Hyun Lee

  6. Department of Emergency Medicine, Institute of Medical Sciences, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea

    Jun Hwi Cho

  7. Oriental Bio-herb Research Institute, Kangwon National University, Chuncheon, 200-701, South Korea

    Myong Jo Kim

  8. Fermentation and Food Processing, Department of Korean Food Research for Globalization, NAAS, RDA, Suwon, 441-707, South Korea

    Tae Young Kim

  9. Department of Food Science and Nutrition, Hallym University, Chuncheon, 200-702, South Korea

    Il-Jun Kang

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  1. Bing Chun Yan
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Correspondence to Il-Jun Kang or Moo-Ho Won.

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Yan, B.C., Park, J.H., Ahn, J.H. et al. Comparison of the Immunoreactivity of Trx2/Prx3 Redox System in the Hippocampal CA1 Region Between the Young and Adult Gerbil Induced by Transient Cerebral Ischemia. Neurochem Res 37, 1019–1030 (2012). https://doi.org/10.1007/s11064-012-0702-8

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  • DOI: https://doi.org/10.1007/s11064-012-0702-8

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