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Neuroprotective Effects of 3α-Acetoxyeudesma-1,4(15),11(13)-trien-12,6α-olide Against Dopamine-Induced Apoptosis in the Human Neuroblastoma SH-SY5Y Cell Line

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Abstract

Dopamine (DA), as a neurotoxin, can elicit severe Parkinson’s disease-like syndrome by elevating intracellular reactive oxygen species (ROS) levels and apoptotic activity. We examined the inhibitory effects of 3α-acetoxyeudesma-1,4(15),11(13)-trien-12,6α-olide (AETO), purified from the leaves of Laurus nobilis L., on DA-induced apoptosis and α-synuclein (α-syn) formation in dopaminergic SH-SY5Y cells. AETO decreased the active form of caspase-3 and the levels of p53, which were accompanied by increased levels of Bcl-2 in a dose-dependent manner. Flow cytometric and Western blot analysis showed that AETO significantly inhibited DA-induced apoptosis along with suppression of intracellular tyrosinase activity, ROS generation, quinoprotein, and α-syn formation (P < 0.01). These results indicate that AETO inhibited DA-induced apoptosis, which is closely related to the suppression of intracellular tyrosinase activity and the formation of α-syn, ROS, and quinoprotein in SH-SY5Y cells.

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Acknowledgments

This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (Grant No. 2009-0086896).

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Authors and Affiliations

  1. Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul, 151-742, Republic of Korea

    Uk Koo, Ahrom Ham, Dahyun Lyu, Bora Kim, Woongchon Mar & Jongheon Shin

  2. Department of Neuroscience, College of Medicine, Korea University, Seoul, 136-705, Republic of Korea

    Kung-Woo Nam

  3. Department of Animal Biotechnology, Kangwon National University, Chuncheon-si, Gangwon, 200-701, Republic of Korea

    Sung-Jin Lee

  4. College of Pharmacy, Kangwon National University, Chuncheon-si, Gangwon, 200-701, Republic of Korea

    Kyeong Ho Kim

  5. Department of Agricultural Biotechnology College of Agriculture and Life Science, Seoul National University, Seoul, 151-921, Republic of Korea

    Ki-Bong Oh

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  1. Uk Koo
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Correspondence to Woongchon Mar or Jongheon Shin.

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The authors U. Koo and Kung-Woo Nam contributed equally to this article. Woongchon Mar and Jongheon Shin are senior co-authors.

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Koo, U., Nam, KW., Ham, A. et al. Neuroprotective Effects of 3α-Acetoxyeudesma-1,4(15),11(13)-trien-12,6α-olide Against Dopamine-Induced Apoptosis in the Human Neuroblastoma SH-SY5Y Cell Line. Neurochem Res 36, 1991–2001 (2011). https://doi.org/10.1007/s11064-011-0523-1

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