Abstract
Previous studies have shown that Csk plays critical roles in the regulation of neural development, differentiation and glutamate-mediated synaptic plasticity. It has been found that Csk associates with the NR2A and 2B subunits of N-methyl-D-aspartate receptors (NMDARs) in a Src activity-dependent manner and serves as an intrinsic mechanism to provide a “brake” on the induction of long-term synaptic potentiation (LTP) mediated by NMDARs. In contrast to the NR2A and 2B subunits, no apparent tyrosine phosphorylation is found in the NR1 subunit of NMDARs. Here, we report that Csk can also associate with the NR1 subunit in a Src activity-dependent manner. The truncation of the NR1 subunit C-tail which contains only one tyrosine (Y837) significantly reduced the Csk association with the NR1-1a/NR2A receptor complex. Furthermore, we found that either the truncation of NR2A C-tail at aa 857 or the mutation of Y837 in the NR1-1a subunit to phenylalanine blocked the inhibition of NR1-1a/NR2A receptors induced by intracellular application of Csk. Thus, both the NR1 and NR2 subunits are required for the regulation of NMDAR activity by Csk.
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Nada S, Okada M, MacAuley A et al (1991) Cloning of a complementary DNA for a protein-tyrosine kinase that specifically phosphorylates a negative regulatory site of p60c-src. Nature 351:69–72
Ogawa A, Takayama Y, Sakai H et al (2002) Structure of the carboxyl-terminal Src kinase, Csk. J Biol Chem 277:14351–14354
Matsuoka H, Nada S, Okada M (2004) Mechanism of Csk-mediated down regulation of Src family tyrosine kinases in EGF signaling. J Biol Chem 279:5975–5983
Cole PA, Shen K, Qiao Y et al (2003) Protein tyrosine kinases Src and Csk: a tail’s tale. Curr Opin Chem Biol 7:580–585
Cohen P (2002) Protein kinases–the major drug targets of the twenty-first century? Nat Rev Drug Discov 1:309–315
Liu XJ, Gingrich JR, Vargas-Caballero M et al (2008) Treatment of inflammatory and neuropathic pain by uncoupling Src from the NMDA receptor complex. Nat Med 14:1325–1332
Ingley E (2008) Src family kinases: regulation of their activities, levels and identification of new pathways. Biochim Biophys Acta 1784:56–65
Imamoto A, Soriano P (1993) Disruption of the csk gene, encoding a negative regulator of Src family tyrosine kinases, leads to neural tube defects and embryonic lethality in mice. Cell 73:1117–1124
Nada S, Yagi T, Takeda H et al (1993) Constitutive activation of Src family kinases in mouse embryos that lack Csk. Cell 73:1125–1135
Dey N, Howell BW, De PK et al (2005) CSK negatively regulates nerve growth factor induced neural differentiation and augments AKT kinase activity. Exp Cell Res 307:1–14
Colognato H, Ramachandrappa S, Olsen IM et al (2004) Integrins direct Src family kinases to regulate distinct phases of oligodendrocyte development. J Cell Biol 167:365–375
Nada S, Shima T, Yanai H et al (2003) Identification of PSD-93 as a substrate for the Src family tyrosine kinase Fyn. J Biol Chem 278:47610–47621
Xu J, Weerapura M, Ali MK et al (2008) Control of excitatory synaptic transmission by C-terminal Src kinase. J Biol Chem 283:17503–17514
Khanna S, Roy S, Park HA et al. (2007, Aug 10) Regulation of c-Src activity in glutamate-induced neurodegeneration. J Biol Chem 282:23482–23490
D’Arco M, Giniatullin R, Leone V et al. (2009, Aug 7) The C-terminal Src inhibitory kinase (Csk)-mediated tyrosine phosphorylation is a novel molecular mechanism to limit P2X3 receptor function in mouse sensory neurons. J Biol Chem 284:21393–21401
Rengifo-Cam W, Konishi A, Morishita N et al (2004) Csk defines the ability of integrin-mediated cell adhesion and migration in human colon cancer cells: implication for a potential role in cancer metastasis. Oncogene 23:289–297
Kawabuchi M, Satomi Y, Takao T et al (2000) Transmembrane phosphoprotein Cbp regulates the activities of Src-family tyrosine kinases. Nature 404:999–1003
Shima T, Nada S, Okada M (2003) Transmembrane phosphoprotein Cbp senses cell adhesion signaling mediated by Src family kinase in lipid rafts. Proc Natl Acad Sci USA 100:14897–14902
Dingledine R, Borges K, Bowie D et al (1999) The glutamate receptor ion channels. Pharmacol Rev 51:7–61
Cheung HH, Gurd JW (2001) Tyrosine phosphorylation of the N-methyl-D-aspartate receptor by exogenous and postsynaptic density-associated Src-family kinases. J Neurochem 78:524–534
Nakazawa T, Komai S, Tezuka T et al (2001) Characterization of Fyn-mediated tyrosine phosphorylation sites on GluRepsilon 2 (NR2B) subunit of the N-Methyl-D-aspartate receptor. J Biol Chem 276:693–699
Yang M, Leonard JP (2001) Identification of mouse NMDA receptor subunit NR2A C-terminal tyrosine sites phosphorylated by coexpression with v-Src. J Neurochem 77:580–588
Monyer H, Sprengel R, Schoepfer R et al (1992) Heteromeric NMDA receptors: molecular and functional distinction of subtypes. Science 256:1217–1221
Karp SJ, Masu M, Eki T et al (1993) Molecular cloning and chromosomal localization of the key subunit of the human N-Methyl-D-Aspartate receptor. J Biol Chem 268:3728–3733
Lau LF, Huganir RL (1995) Differential tyrosine phosphorylation of N-methyl-D-aspartate receptor subunits. J Biol Chem 270:20036–20041
Vissel B, Krupp JJ, Heinemann SF et al (2001) A use-dependent tyrosine dephosphorylation of NMDA receptors is independent of ion flux. Nat Neurosci 4:587–596
Polte TR, Hanks SK (1997) Complexes of focal adhesion kinase (FAK) and Crk-associated substrate (p130(Cas)) are elevated in cytoskeleton-associated fractions following adhesion and Src transformation. Requirements for Src kinase activity and FAK proline-rich motifs. J Biol Chem 272:5501–5509
Lei G, Xue S, Chery N et al (2002) Gain control of N-methyl-D-aspartate receptor activity by receptor-like protein tyrosine phopshatase alpha. EMBO J 21:2977–2989
Karni R, Mizrachi S, Reiss-Sklan E et al (2003) The pp60c-Src inhibitor PP1 is non-competitive against ATP. FEBS Lett 537:47–52
Bain J, McLauchlan H, Elliott M et al (2003) The specificities of protein kinase inhibitors: an update. Biochem J 371:199–204
Hsueh RC, Scheuermann RH (2000) Tyrosine kinase activation in the decision between growth, differentiation, and death responses initiated from the B cell antigen receptor. Adv Immunol 75:283–316
Kalia LV, Gingrich JR, Salter MW (2004) Src in synaptic transmission and plasticity. Oncogene 23:8007–8016
Acknowledgments
This work was supported by a grant from NIH (5R01 NS053567-04) to XMY. SF is supported by the State Scholarship Fund of China (2009845013). We also thank Dr. J. Olcese for his technical help.
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Xiao-Qian Fang and Jindong Xu contributed to this work equally.
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Fang, XQ., Xu, J., Feng, S. et al. The NMDA Receptor NR1 Subunit is Critically Involved in the Regulation of NMDA Receptor Activity by C-terminal Src kinase (Csk). Neurochem Res 36, 319–326 (2011). https://doi.org/10.1007/s11064-010-0330-0
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DOI: https://doi.org/10.1007/s11064-010-0330-0