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Salvianolic Acid A Exerts Antiamnesic Effect on Diazepam-Induced Anterograde Amnesia in Mice

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Abstract

Benzodiazepine was known to produce amnesia. Salvianolic acid A extracted from Salvia miltiorrhiza was an effective antioxidant. The objective of the study was to evaluate the effect of salvianolic acid A on diazepam-induced amnesia in mice. C57BL/6 mice were treated with salvianolic acid A at doses of 10, 20 and 40 mg/kg following administration with diazepam at a dose of 3 mg/kg. Morris water maze was performed to evaluate the effect of salvianolic acid A on amnesia. The antioxidative parameters in hippocampus were measured. The results showed that salvianolic acid A decreased the mean escape latency and increased the percentage of time spent in target quadrant. Salvianolic acid A reduced the content of malondialdehyde and increased the activities of superoxide dismutase, catalase and glutathione peroxidase in hippocampus. The findings demonstrated that salvianolic acid A had antiamnesic effects on diazepam-induced anterograde amnesia in mice, by augmenting the antioxidative capacity of hippocampus.

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References

  1. Mejo SL (1992) Anterograde amnesia linked to benzodiazepines. Nurse Pract 17:49–50

    Article  Google Scholar 

  2. Ghoneim MM, Hinrichs JV, Mewaldt SP (1984) Dose–response analysis of the behavioral effects of diazepam: I. Learning and memory. Psychopharmacology (Berl) 82:291–295

    Article  CAS  Google Scholar 

  3. Tomaz C, Dickinson-Anson H, McGaugh JL (1992) Basolateral amygdala lesions block diazepam-induced anterograde amnesia in an inhibitory avoidance task. Proc Natl Acad Sci USA 89:3615–3619

    Article  CAS  PubMed  Google Scholar 

  4. White AM, Simson PE, Best PJ (1997) Comparison between the effects of ethanol and diazepam on spatial working memory in the rat. Psychopharmacology (Berl) 133:256–261

    Article  CAS  Google Scholar 

  5. Prabhakar S, Saraf MK, Pandhi P, Anand A (2008) Bacopa monniera exerts antiamnesic effect on diazepam-induced anterograde amnesia in mice. Psychopharmacology (Berl) 200:27–37

    Article  CAS  Google Scholar 

  6. Olkkola KT, Ahonen J (2008) Midazolam and other benzodiazepines. Handb Exp Pharmacol 182:335–360

    Article  CAS  PubMed  Google Scholar 

  7. Kim DH, Jeon SJ, Son KH, Jung JW, Lee S, Yoon BH et al (2007) The ameliorating effect of oroxylin A on scopolamine-induced memory impairment in mice. Neurobiol Learn Mem 87:536–546

    Article  CAS  PubMed  Google Scholar 

  8. Thiebot MH (1985) Some evidence for amnesic-like effects of benzodiazepines in animals. Neurosci Biobehav Rev 9:95–100

    Article  CAS  PubMed  Google Scholar 

  9. Ghoneim MM, Dembo JB, Block RI (1989) Time course of antagonism of sedative and amnesic effects of diazepam by flumazenil. Anesthesiology 70:899–904

    Article  CAS  PubMed  Google Scholar 

  10. Curran HV, Birch B (1991) Differentiating the sedative, psychomotor and amnesic effects of benzodiazepines: a study with midazolam and the benzodiazepine antagonist, flumazenil. Psychopharmacology (Berl) 103:519–523

    Article  CAS  Google Scholar 

  11. Musavi S, Kakkar P (1998) Diazepam induced early oxidative changes at the subcellular level in rat brain. Mol Cell Biochem 178:41–46

    Article  CAS  PubMed  Google Scholar 

  12. Halliwell B (1992) Reactive oxygen species and the central nervous system. J Neurochem 59:1609–1623

    Article  CAS  PubMed  Google Scholar 

  13. Liu P, Hu Y, Liu C, Liu C, Zhu D (2001) Effects of salviainolic acid A (SA-A) on liver injury: SA-A action on hepatic peroxidation. Liver 21:384–390

    Article  CAS  PubMed  Google Scholar 

  14. Wu ZM, Wen T, Tan YF, Liu Y, Ren F, Wu H (2007) Effects of salvianolic acid a on oxidative stress and liver injury induced by carbon tetrachloride in rats. Basic Clin Pharmacol Toxicol 100:115–120

    Article  CAS  PubMed  Google Scholar 

  15. Du G, Zhang J (1997) Protective effects of salvianolic acid A against impairment of memory induced by cerebral ischemia-reperfusion in mice. Chin Med J (Engl) 110:65–68

    CAS  Google Scholar 

  16. Morris R (1984) Developments of a water-maze procedure for studying spatial learning in the rat. J Neurosci Methods 11:47–60

    Article  CAS  PubMed  Google Scholar 

  17. Malleret G, Hen R, Guillou JL, Segu L, Buhot MC (1999) 5-HT1B receptor knock-out mice exhibit increased exploratory activity and enhanced spatial memory performance in the Morris water maze. J Neurosci 19:6157–6168

    CAS  PubMed  Google Scholar 

  18. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    CAS  PubMed  Google Scholar 

  19. Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358

    Article  CAS  PubMed  Google Scholar 

  20. McCord JM, Fridovich I (1969) Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J Biol Chem 244:6049–6055

    CAS  PubMed  Google Scholar 

  21. Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126

    Article  CAS  PubMed  Google Scholar 

  22. Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–169

    CAS  PubMed  Google Scholar 

  23. Lister RG (1985) The amnesic action of benzodiazepines in man. Neurosci Biobehav Rev 9:87–94

    Article  CAS  PubMed  Google Scholar 

  24. Behl C, Moosmann B (2002) Antioxidant neuroprotection in Alzheimer’s disease as preventive and therapeutic approach. Free Radic Biol Med 33:182–191

    Article  CAS  PubMed  Google Scholar 

  25. Ravindranath V, Boyd MR (1995) Xenobiotic metabolism in brain. Drug Metab Rev 27:419–448

    Article  CAS  PubMed  Google Scholar 

  26. Darragh A, Lambe R, Kenny M, Brick I, Taaffe W, O’Boyle C (1982) Ro 15–1788 antagonises the central effects of diazepam in man without altering diazepam bioavailability. Br J Clin Pharmacol 14:677–682

    CAS  PubMed  Google Scholar 

  27. Dorow R, Berenberg D, Duka T, Sauerbrey N (1987) Amnestic effects of lormetazepam and their reversal by the benzodiazepine antagonist Ro 15–1788. Psychopharmacology 93:507–514

    Article  CAS  PubMed  Google Scholar 

  28. Gentil V, Tavares S, Gorenstein C, Bello C, Mathias L, Gronich G, Singer J (1990) Acute reversal of flunitrazepam effects by Ro 15–1788 and Ro 15–3505: inverse agonism, tolerance, and rebound. Psychopharmacology 100:54–59

    Article  CAS  PubMed  Google Scholar 

  29. Musavi S, Kakkar P (2000) Pro and antioxidant responses to repeated administration of diazepam in rat brain. Mol Cell Biochem 206:97–103

    Article  CAS  PubMed  Google Scholar 

  30. Venault P, Chapouthier G, de Carvalho LP, Simiand J, Morre M, Dodd RH et al (1986) Benzodiazepine impairs and beta-carboline enhances performance in learning and memory tasks. Nature 321:864–866

    Article  CAS  PubMed  Google Scholar 

  31. McNamara RK, dePape GE, Skelton RW (1993) Differential effects of benzodiazepine receptor agonists on hippocampal long-term potentiation and spatial learning in the Morris water maze. Brain Res 626:63–70

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This work was supported by the Natural Science Foundation of Shandong Province (No. ZR2009CQ004), and International S&T Cooperation Program of China (2009DFA31100). The authors also thank Prof. Dr. Lon Clark for the English language revision.

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Authors and Affiliations

  1. Department of Pharmacology, School of Pharmacy, Yantai University, 32#, Qingquan Road, Yantai, 264005, People’s Republic of China

    T. Wang, B. Han, L. M. Zhang & F. H. Fu

  2. School of Foreign Languages, Yantai University, 32#, Qingquan Road, Yantai, 264005, People’s Republic of China

    S. Y. Shan

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  1. T. Wang
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  2. S. Y. Shan
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Correspondence to F. H. Fu.

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Wang, T., Shan, S.Y., Han, B. et al. Salvianolic Acid A Exerts Antiamnesic Effect on Diazepam-Induced Anterograde Amnesia in Mice. Neurochem Res 36, 103–108 (2011). https://doi.org/10.1007/s11064-010-0270-8

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  • DOI: https://doi.org/10.1007/s11064-010-0270-8

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