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Cornel Iridoid Glycoside Inhibits Inflammation and Apoptosis in Brains of Rats with Focal Cerebral Ischemia

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Abstract

The capacity of cornel iridoid glycoside (CIG) to suppress the manifestations of ischemic stroke was investigated. CIG was administered to rats by the intragastric route once daily for 7 days. Focal cerebral ischemia was induced by 2 h of middle cerebral artery occlusion followed by 24 h of reperfusion. In non-treated rats large infarct areas were observed within 24 h of reperfusion. Examination of the ischemic cerebral cortex revealed microglia and astrocyte activation, increased interleukin-1β (IL-1 β) and tumor necrosis factor-α (TNF-α) concentrations, increased DNA fragmentation in the ischemia penumbra, elevated Bax expression, increased caspase-3 cleavage, and decreased Bcl-2 expression. Pretreatment with CIG decreased the infarct area, DNA fragmentation, IL-1β and TNF-α concentrations, microglia and astrocyte activation, Bax expression, and caspase-3 cleavage while increasing Bcl-2 expression. CIG exerts anti-neuroinflammatory and anti-apoptotic effects which should prove beneficial for prevention or treatment of stroke.

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Acknowledgments

This study was supported by the National Key Basic Research 973 Program of China (Grant No. 2003CB517104), the National Natural Science Foundation of China (Grant No. 90709011, 30973513), and the Beijing Municipal Science and Technology Program of China (Grant No. D0206001043191).

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Authors and Affiliations

  1. Department of Pharmacology, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Xuanwu Hospital of Capital Medical University, 45 Chang-chun Street, 100053, Beijing, People’s Republic of China

    Bai-liu Ya, Lan Zhang, Wen Wang & Lin Li

  2. Department of Neurology, Affiliated Hospital of Inner Mongolia Medical College, 010059, Hohhot, Inner Mongolia, People’s Republic of China

    Chun-yang Li

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  1. Bai-liu Ya
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  2. Chun-yang Li
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  3. Lan Zhang
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  4. Wen Wang
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  5. Lin Li
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Correspondence to Lin Li.

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Ya, Bl., Li, Cy., Zhang, L. et al. Cornel Iridoid Glycoside Inhibits Inflammation and Apoptosis in Brains of Rats with Focal Cerebral Ischemia. Neurochem Res 35, 773–781 (2010). https://doi.org/10.1007/s11064-010-0134-2

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  • DOI: https://doi.org/10.1007/s11064-010-0134-2

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