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Inhibitory Effect of Antioxidants on Hydroxyl Radical Generation from Methylguanidine: An ESR Study

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Abstract

Methylguanidine (MG) is known as not only a nephrotoxin but also as a neurotoxine. We have previously showed that MG itself generates hydroxyl radicals (•OH) in an in vitro study. In this study, we examined the inhibitory effects of ascorbate, EPC-K1 (α-tocopheryl-l-ascorbate-2-O-phosphate diester), Trolox (water-soluble vitamin E analogue), and glutathione (GSH) on •OH generation from MG using an electron spin resonance (ESR) spectrometry with spin trap 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). It was found that these compounds have potent inhibitory effect on •OH generation from MG in the order of ascorbate > GSH > EPC-K1 > Trolox.

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Abbreviations

DMPO:

5,5-Dimethyl-1-pyrroline-N-oxide

DMSO:

Dimethylsulfoxide

DMPO-OH:

DMPO-spin adducts of hydroxyl radicals

EPC-K1 :

α-Tocopheryl-l-ascorbate-2-O-phosphate diester

ESR:

Electron spin resonance

GSH:

Reduced glutathione

•OH:

Hydroxyl radicals

MnO:

Manganese oxide

MG:

Methylguanidine

ROS:

Reactive oxygen species

R.P.H.:

Relative peak height

O2 •− :

Superoxide anion radicals

SOD:

Superoxide dismutase

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Authors and Affiliations

  1. Department of Anti-aging Food Sciences, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Okayama, 700-8558, Japan

    Yasuko Noda & Mitsumasa Mankura

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  1. Yasuko Noda
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  2. Mitsumasa Mankura
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Correspondence to Yasuko Noda.

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Special issue article in honor of Dr. Akitane Mori.

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Noda, Y., Mankura, M. Inhibitory Effect of Antioxidants on Hydroxyl Radical Generation from Methylguanidine: An ESR Study. Neurochem Res 34, 734–738 (2009). https://doi.org/10.1007/s11064-008-9811-9

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