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Protective Effects of Nigella sativa on the Neuronal Injury in Frontal Cortex and Brain Stem After Chronic Toluene Exposure

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Abstract

The goal of this study was designed to evaluate the possible protective effects of Nigella sativa (NS) on the neuronal injury in the frontal cortex and brain stem after chronic toluene exposure in rats. The rats were randomly alotted into one of three experimental groups: A (control), B (toluene treated) and C (toluene treated with NS); each group contain 10 animals. Control group received 1 ml serum physiologic and toluene treatment was performed by inhalation of 3,000 ppm toluene, in a 8 h/day and 6 day/week order for 12 weeks. The rats in NS treated group was given NS (in a dose of 400 mg/kg body weight) once a day orally by using intra gastric intubation for 12 weeks starting just after toluene exposure. Tissue samples were obtained for histopathological investigation. To date, no histopathological changes of neurodegeneration in the frontal cortex and brain stem after chronic toluene exposure in rats by NS treatment have been reported. In this study, chronic toluene exposure caused severe degenerative changes, shrunken cytoplasma, severely dilated cisternae of endoplasmic reticulum, markedly swollen mitochondria with degenerated cristae and nuclear membrane breakdown with chromatin disorganization in neurons of the frontal cortex and brain stem. The nerve cells showing the pathologic changes were almost absent in the NS-treated rats. We conclude that NS therapy causes morphologic improvement on neurodegeneration in frontal cortex and brain stem after chronic toluene exposure in rats. We believe that further preclinical research into the utility of NS may indicate its usefulness as a potential treatment on neurodegeneration after chronic toluene exposure in rats.

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References

  1. Bergamaschi E, Smargiassi A, Mutti A, Cavazzini S, Vettori MV, Alinovi R, Franchini I, Mergler D (1997) Peripheral markers of catecholaminergic dysfunction and symptoms of neurotoxicity among styrene-exposed workers. Int Arch Occup Environ Health 69(3):209–214

    Article  PubMed  CAS  Google Scholar 

  2. Benignus VA, Muller KE, Barton CN, Bittikofer JA (1981) Toluene levels in blood and brain of rats during and after respiratory exposure. Toxicol Appl Pharmacol 61(3):326–334

    Article  PubMed  CAS  Google Scholar 

  3. Greenberg MM (1997) The central nervous system and exposure to toluene: a risk characterization. Environ Res 72(1):1–7

    Article  PubMed  CAS  Google Scholar 

  4. Pryor GT, Rebert CS, Howd RA (1987) Hearing loss in rats caused by inhalation of mixed xylenes and styrene. J Appl Toxicol 7:55–61

    Article  PubMed  CAS  Google Scholar 

  5. Crofton KM, Lassiter TL, Rebert CS (1994) Solvent-induced ototoxicity in rats: an atypical selective mid-frequency hearing deficit. Hear Res 80(1):25–30

    Article  PubMed  CAS  Google Scholar 

  6. Campagna D, Stengel B, Mergler D, Limasset JC, Diebold F, Michard D, Huel G (2001) Color vision and occupational toluene exposure. Neurotoxicol Teratol 23(5):473–480

    Article  PubMed  CAS  Google Scholar 

  7. Baelum J (1991) Human solvent exposure: factors influencing the pharmacokinetics and acute toxicity. Pharmacol Toxicol 1:1–36

    Google Scholar 

  8. Ladefoged O, Strange P, Moller A, Lam HR, Ostergaard G, Larsen JJ, Arlien-Soborg P (1991) Irreversible effects in rats of toluene (inhalation) exposure for six months. Pharmacol Toxicol 5:384–390

    Google Scholar 

  9. Hass U, Lund SP, Hougaard KS, Simonsen L (1999) Developmental neurotoxicity after toluene inhalation exposure in rats. Neurotoxicol Teratol 21:349–357

    Article  PubMed  CAS  Google Scholar 

  10. Mattia CJ, Ali SF, Bondy SC (1993) Toluene-induced oxidative stres in several brain regions and other organs. Mol Chem Neuropathol 3:313–328

    Google Scholar 

  11. Burmistrov SO, Arutyunyan AV, Stepanov MG, Oparina TI, Prokopenko VM (2001) Effect of chronic inhalation of toluene and dioxane on activity of free radical processes in rat ovaries and brain. Bull Exp Biol Med 3:832–836

    Article  Google Scholar 

  12. Benignus VA (1981) Neurobehavioral effects of toluene: a review. Neurobehav Toxicol Teratol 3:407–415

    PubMed  CAS  Google Scholar 

  13. Oppenheim RW (1991) Cell death during development of the nervous system. Annu Rev Neurosci 14:453–501

    Article  PubMed  CAS  Google Scholar 

  14. Oppenheim RW (1996) The concept of uptake and retrograde transport of neurotrophic molecules during development: history and present status. Neurochem Res 21:769–777

    Article  PubMed  CAS  Google Scholar 

  15. Clarke PG, Posada A, Primi MP, Castagne V (1998) Neuronal death in the central nervous system during development. Biomed Pharmacother 52:356–362

    Article  PubMed  CAS  Google Scholar 

  16. Zimmermann KC, Bonzon C, Green DR (2001) The machinery of programmed cell death. Pharmacol Ther 92:57–70

    Article  PubMed  CAS  Google Scholar 

  17. Cohen GM (1997) Caspases: the executioners of apoptosis. Biochem J 326:1–16

    PubMed  CAS  Google Scholar 

  18. Henderson Z (1996) Responses of basal forebrain cholinergic neurons to damage in the adult brain. Prog Neurobiol 48:219–254

    Article  PubMed  CAS  Google Scholar 

  19. Hörtnagl H, Hellweg R (1997) Insights into the role of the cholinergic component of the septohippocampal pathway: what have we learned from experimental lesion studies? Brain Res Bull 43:245–255

    Article  PubMed  Google Scholar 

  20. El-Tahir KE, Ashour MM, Al-Harbi MM (1993) The respiratory effects of the volatile oil of the black seed (Nigella sativa) in guinea-pigs: elucidation of the mechanism(s) of action. Gen Pharmacol 24:1115–1122

    PubMed  CAS  Google Scholar 

  21. El-Kadi A, Kandil O (1987) The black seed (Nigella sativa) and immunity: its effect on human T cell subset. Fed Proc 46:1222

    Google Scholar 

  22. Hanafy MSM, Hatem ME (1991) Studies on the antimicrobial activity of Nigella sativa seed (black cumin). J Ethnopharmacol 34:275–278

    Article  PubMed  CAS  Google Scholar 

  23. Zaoui A, Cherrah Y, Lacaille-Dubois MA, Settaf A, Amarouch H, Hassar M (2000) Diuretic and hypotensive effects of Nigella sativa in the spontaneously hypertensive rat. Therapie 55:379–382

    PubMed  CAS  Google Scholar 

  24. Kanter M, Meral I, Yener Z, Ozbek H, Demir H (2003) Partial regeneration/proliferation of the β-Cells in the islets of Langerhans by Nigella sativa L in streptozocin-induced diabetic rats. Tohoku J Exp Med 20:213–219

    Article  Google Scholar 

  25. Kanter M, Coskun O, Korkmaz A, Oter S (2004) Effects of Nigella sativa on oxidative stress and β-Cell damage in streptozocin-induced diabetic rats. Anat Rec 279:685–691

    Article  CAS  Google Scholar 

  26. Kanter M, Coskun O, Budancamanak M (2005) Hepatoprotective effects of Nigella sativa L and Urtica dioica L on lipid peroxidation, antioxidant enzyme systems and liver enzymes in carbon tetrachloride-treated rats. World J Gastroenterol 11(42):6684–6688

    PubMed  Google Scholar 

  27. Kanter M, Demir H, Karakaya C, Ozbek H (2005) Gastroprotective activity of Nigella sativa L oil and its constituent, thymoquinone against acute alcohol-induced gastric mucosal injury in rats. World J Gastroenterol 11(42):6662–6666

    PubMed  Google Scholar 

  28. Kanter M, Coskun O, Uysal H (2006) The antioxidative and antihistaminic effect of Nigella sativa and its major constituent, thymoquinone on ethanol-induced gastric mucosal damage. Arch Toxicol 80(4):217–224

    Article  PubMed  CAS  Google Scholar 

  29. Kanter M, Coskun O, Kalayci M, Buyukbas S, Cagavi F (2006) Neuroprotective effects of Nigella sativa on experimental spinal cord injury in rats. Hum Exp Toxicol 25(3):127–133

    Article  PubMed  CAS  Google Scholar 

  30. Fararh KM, Atoji Y, Shimizu Y, Takewaki T (2002) Isulinotropic properties of Nigella sativa oil in streptozotocin plus nicotinamide diabetic hamster. Res Vet Sci 73:279–282

    Article  PubMed  CAS  Google Scholar 

  31. Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577–580

    PubMed  CAS  Google Scholar 

  32. Balster RL (1998) Neural basis of inhalant abuse. Drug Alcohol Depend 51:207–214

    Article  PubMed  CAS  Google Scholar 

  33. Moser VC, Balster RL (1986) The effects of inhaled toluene, halothane, 1, 1, 1,-trichloroethane, and ethanol on fixed-interval responding in mice. Neurobehav Toxicol Teratol 8(5):525–531

    PubMed  CAS  Google Scholar 

  34. Warren DA, Bowen SE, Jennings WB, Dallas CE, Balster RL (2000) Biphasic effects of 1, 1, 1-trichloroethane on the locomotor activity of mice: relationship to blood and brain solvent concentrations. Toxicol Sci 56(2):365–373

    Article  PubMed  CAS  Google Scholar 

  35. Tegeris JS, Balster RL (1994) A comparison of the acute behavioral effects of alkylbenzenes using a functional observational battery in mice. Fundam Appl Toxicol 22(2):240–250

    Article  PubMed  CAS  Google Scholar 

  36. Echeverria D, Fine L, Langolf G, Schork T, Sampaio C (1991) Acute behavioural comparisons of toluene and ethanol in human subjects. Br J Ind Med 48:750–761

    PubMed  CAS  Google Scholar 

  37. Mattia CJ, Adams JD Jr, Bondy SC (1993) Free radical induction in the brain and liver by products of toluene catabolism. Biochem Pharmacol 46(1):103–110

    Article  PubMed  CAS  Google Scholar 

  38. Zabedah MY, Razak M, Zakiak I, Zuraidah AB (2001) Profile of solvent abusers (glue sniffers) in East Malaysia. Malays J Pathol 23(2):105–109

    PubMed  CAS  Google Scholar 

  39. Gotohda T, Tokunaga I, Kubo S, Morita K, Kitamura O, Eguchi A (2000) Effect of toluene inhalation on astrocytes and neurotrophic factor in rat brain. Forensic Sci Int 11:233–238

    Article  Google Scholar 

  40. Moonen G, Rogister B, Leprince P, Rigo JM, Delree P, Lefebvre PP, Schoenen J (1990) Neuro-glial interactions and neural plasticity. Prog Brain Res 86:63–73

    Article  PubMed  CAS  Google Scholar 

  41. Papadopoulos MC, Koumenis IL, Yuan TY, Giffard RG (1999) Primary cortical glial reaction versus secondary thalamic glial response in the excitotoxically injured young brain: astroglial response and metallothionein expression. Neuroscience 92:827–839

    Article  Google Scholar 

  42. Altenkirch H, Mager J, Stoltenburg G, Helmbrecht J (1977) Toxic polyneuropathies after sniffing a glue thinner. J Neurol 214:137–52

    Article  PubMed  CAS  Google Scholar 

  43. Coskun O, Yuncu M, Kanter M, Buyukbas S (2006) Ebselen protects against oxidative and morphological effects of high concentration chronic toluene exposure on rat sciatic nerves. Eur J Gen Med 3(2):64–72

    CAS  Google Scholar 

  44. Saavedra H, Palestini M, De Marinis A (1996) Neuronal changes induced by chronic toluene exposure in the cat. Arch Ital Biol 134(3):217–225

    PubMed  CAS  Google Scholar 

  45. Korbo L, Ladefoged O, Lam HR, Ostergaard G, West MJ, Arlien- Soborg P (1996) Neuronal loss in hippocampus in rats exposed to toluene. Neurotoxicology 17:359–366

    PubMed  CAS  Google Scholar 

  46. Baydas G, Reiter RJ, Nedzvetskii VS, Yasar A, Tuzcu M, Ozveren F, Canatan H (2003) Melatonin protects the central nervous system of rats against toluene-containing thinner intoxication by reducing reactive gliosis. Toxicol Lett 137(3):169–174

    Article  PubMed  CAS  Google Scholar 

  47. Chen ST, Chuang JI, Hong MH, Li EIC (2002) Melatonin attenuates MPP+-induced neurodegeneration and glutathione impairment in the nigrostriatal dopaminergic pathway. J Pineal Res 32:262–269

    Article  PubMed  CAS  Google Scholar 

  48. Shen YX, Xu SY, Wei W, Sun XX, Liu LH, Yang J, Dong C (2002) The protective effects of melatonin from oxidative damage induced by amyloid beta-peptide 25–35 in middle aged rats. J Pineal Res 32:85–89

    Article  PubMed  CAS  Google Scholar 

  49. Cuzzocrea S, Reiter RJ (2001) Pharmacological action of melatonin in shock, inflammation and ischemia/reperfusion injury. Eur J Pharmacol 426:1–10

    Article  PubMed  CAS  Google Scholar 

  50. Kanter M (2007) Nigella sativa and derived thymoquinone prevents hippocampal neurodegeneration after chronic toluene exposure in rats. Neurochem Res 33(3):579–588

    Article  PubMed  CAS  Google Scholar 

  51. Yuan J, Yankner BA (2000) Apoptosis in the nervous system. Nature 407:802–809

    Article  PubMed  CAS  Google Scholar 

  52. Oltvai ZN, Milliman CL, Korsmeyer SJ (1993) Bcl–2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 74:609–619

    Article  PubMed  CAS  Google Scholar 

  53. Dalgaard M, Hossaini A, Hougaard KS, Hass U, Ladefoged O (2001) Developmental toxicity of toluene in male rats: effects on semen quality, testes morphology, and apoptotic neurodegeneration. Arch Toxicol 75:103–109

    Article  PubMed  CAS  Google Scholar 

  54. Ladefoged O, Hougaard KS, Hass U, Sørensen IK, Lund SP (2004) Gitte winkel svendsen and Henrik rye lam, effects of combined prenatal stress and toluene exposure on apoptotic neurodegeneration in cerebellum and hippocampus of rats. Basic Clin Pharmacol Toxicol 94:169–176

    PubMed  CAS  Google Scholar 

  55. Wen X, Huang Y, Wang J (2006) Erythropoietin preconditioning on hippocampus neuronal apoptosis following status epilepticus induced by Li-pilocarpine in rats through anti-caspase-3 expression. Neurol India 54(1):58–63

    Article  PubMed  Google Scholar 

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  1. Department of Histology and Embryology, Faculty of Medicine, Trakya University, Edirne, Turkey

    Mehmet Kanter

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Kanter, M. Protective Effects of Nigella sativa on the Neuronal Injury in Frontal Cortex and Brain Stem After Chronic Toluene Exposure. Neurochem Res 33, 2241–2249 (2008). https://doi.org/10.1007/s11064-008-9702-0

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