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Potassium permeability of voltage-operated calcium channels of dorsal root ganglion neurons in a calcium-free medium

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Abstract

In freshly isolated neurons of the rat spinal ganglia, we studied the behavior of voltage-operated calcium channels of these cells under conditions of the absence of calcium ions in the extracellular solution; a patch-clamp technique in the whole-cell configuration was used. We found that such channels in a part of the studied neurons lose their selectivity in a calcium-free potassium-containing solution and become capable of passing an inward potassium current. This current was inhibited by blockers of voltage-operated calcium channels, nifedipine and nickel, and also was to some extent inhibited by caffeine. The latter effect is realized, perhaps, due to calcium-dependent inactivation of calcium channels induced by the action of calcium ions released from the endoplasmic reticulum upon caffeine-induced activation of ryanodine receptors. The peculiarities of current-voltage relationships and characteristics of activation/inactivation of calcium channels modified in calcium-free medium and the possible mechanisms of such modification are discussed.

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Authors and Affiliations

  1. International Center for Molecular Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine

    S. V. Korol’, T. Yu. Korol’ & P. G. Kostyuk

  2. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine

    E. P. Kostyuk & P. G. Kostyuk

Authors
  1. S. V. Korol’
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  2. T. Yu. Korol’
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  3. E. P. Kostyuk
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  4. P. G. Kostyuk
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Corresponding author

Correspondence to S. V. Korol’.

Additional information

Neirofiziologiya/Neurophysiology, Vol. 40, No. 2, pp. 93–99, March–April, 2008.

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Korol’, S.V., Korol’, T.Y., Kostyuk, E.P. et al. Potassium permeability of voltage-operated calcium channels of dorsal root ganglion neurons in a calcium-free medium. Neurophysiology 40, 79–85 (2008). https://doi.org/10.1007/s11062-008-9029-0

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  • DOI: https://doi.org/10.1007/s11062-008-9029-0

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