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Radiolabeled cyclic arginine-glycine-aspartic (RGD)-conjugated iron oxide nanoparticles as single-photon emission computed tomography (SPECT) and magnetic resonance imaging (MRI) dual-modality agents for imaging of breast cancer

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Abstract

Ultrasmall superparamagnetic iron oxide nanoparticles (USPIOs) modified with a novel cyclic arginine-glycine-aspartate (RGD) peptide were made and radiolabeled as single-photon emission computed tomography (SPECT) and magnetic resonance imaging (MRI) dual-modality agents for imaging of breast cancer. The probe was tested both in vitro and in vivo to determine its receptor targeting efficacy and feasibility for SPECT and MRI. The radiochemical syntheses of 125I-cRGD-USPIO were accomplished with a radiochemical purity of 96.05 ± 0.33 %. High radiochemical stability was found in fresh human serum and in phosphate-buffered saline. The average hydrodynamic size of 125I-cRGD-USPIO determined by dynamic light scattering was 51.3 nm. Results of in vitro experiments verified the specificity of the radiolabeled nanoparticles to tumor cells. Preliminary biodistribution studies of 125I-radiolabeled cRGD-USPIO in Bcap37-bearing nude mice showed that it had long circulation half-life, high tumor uptake, and high initial blood retention with moderate liver uptake. In vivo tumor targeting and uptake of the radiolabeled nanoparticles in mice model were visualized by SPECT and MRI collected at different time points. Our results strongly indicated that the 125I-cRGD-USPIO could be used as a promising bifunctional radiotracer for early clinical tumor detection with high sensitivity and high spatial resolution by SPECT and MRI.

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Authors and Affiliations

  1. Department of Nuclear Medicine, The First Affiliated Hospital of Soochow University, Suzhou, China

    Shengming Deng, Wei Zhang, Bin Zhang, Qing Chen, Jiajia Dong, Zhiqiang Chen & Yiwei Wu

  2. School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China

    Shengming Deng

  3. Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, China

    Bin Zhang

  4. College of Chemistry, Chemical Engineering and Materials Science & Key Laboratory of Organic Synthesis of Jiangsu Province, Soochow University, Suzhou, China

    Ruoyu Hong

  5. Department of Radiology, The First Affiliated Hospital of Soochow University, Suzhou, China

    Yinyiin Chen

Authors
  1. Shengming Deng
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  2. Wei Zhang
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  3. Bin Zhang
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  4. Ruoyu Hong
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  5. Qing Chen
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  6. Jiajia Dong
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  7. Yinyiin Chen
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  8. Zhiqiang Chen
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  9. Yiwei Wu
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Correspondence to Bin Zhang or Yiwei Wu.

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Deng, S., Zhang, W., Zhang, B. et al. Radiolabeled cyclic arginine-glycine-aspartic (RGD)-conjugated iron oxide nanoparticles as single-photon emission computed tomography (SPECT) and magnetic resonance imaging (MRI) dual-modality agents for imaging of breast cancer. J Nanopart Res 17, 19 (2015). https://doi.org/10.1007/s11051-014-2845-9

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  • DOI: https://doi.org/10.1007/s11051-014-2845-9

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