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Cisplatin and quantum dots encapsulated in liposomes as multifunctional nanocarriers for theranostic use in brain and skin

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Abstract

We aimed to develop liposomes for loading both cisplatin and quantum dots (QDs) for both drug delivery and bioimaging. The resultant quantum-dot-liposomes (QDLs) with cisplatin were characterized using dynamic light scattering, transmission electron microscopy (TEM), encapsulation efficiencies, and fluorescence intensity. QDLs composed of CdSe or CdSe/ZnS QDs represented a size of about 100 nm. The QDLs were prepared at a high QD loading efficiency of nearly 100 %. Most QDs were located within the liposomal bilayers as evidenced by TEM. Slow and sustained cisplatin release from QDLs was achieved. The cellular uptake of QDLs demonstrated effective internalization and significant fluorescence in melanoma cells. The signal derived from QDLs could be observed by different wavelength settings. The cisplatin-containing QDLs revealed higher cytotoxic activity compared to an equal dose of free cisplatin. CdSe/ZnS QDLs were intravenously administered to mice, and the biodistribution was observed with an in vivo imaging system. Significant fluorescence signal and cisplatin accumulation were detected in the brain and skin, as verified by ex vivo imaging and drug distribution. Liposomal inclusion could reduce the reticuloendothelial system uptake of QDs and cisplatin. QDLs evaluated in this study represent a new potential method for theranostic purposes.

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Acknowledgment

This project was supported by the National Plan for Science and Technology in the kingdom of Saudi Arabia (grant number: 10-NAN1030-02).

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Authors and Affiliations

  1. Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan, 333, Taiwan

    Li-Wen Zhang & Jia-You Fang

  2. School of Medicine, Chang Gung University, Kweishan, Taoyuan, Taiwan

    Chih-Jen Wen

  3. Center for Vascularized Composite Allotransplantation, Chang Gung Memorial Hospital, Kweishan, Taoyuan, Taiwan

    Chih-Jen Wen

  4. Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia

    Saleh A. Al-Suwayeh

  5. Molecular Imaging Center, Chang Gung Memorial Hospital, Kweishan, Taoyuan, Taiwan

    Tzu-Chen Yen

  6. Department of Cosmetic Science, Chang Gung University of Science and Technology, Kweishan, Taoyuan, Taiwan

    Jia-You Fang

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  1. Li-Wen Zhang
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  2. Chih-Jen Wen
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  3. Saleh A. Al-Suwayeh
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Correspondence to Jia-You Fang.

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Li-Wen Zhang and Chih-Jen Wen contributed equally to this work.

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Zhang, LW., Wen, CJ., Al-Suwayeh, S.A. et al. Cisplatin and quantum dots encapsulated in liposomes as multifunctional nanocarriers for theranostic use in brain and skin. J Nanopart Res 14, 882 (2012). https://doi.org/10.1007/s11051-012-0882-9

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  • DOI: https://doi.org/10.1007/s11051-012-0882-9

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