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Resistance Mechanism in a Terbinafine-Resistant Strain of Microsporum canis

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A Correction to this article was published on 29 January 2018

This article has been updated

Abstract

To clarify the terbinafine (TRF) resistance mechanism in a TRF-resistant strain of Microsporum canis, the expression of the pleiotropic drug resistance (PDR1), multidrug resistance (MDR1), MDR2 and MDR4 genes were investigated by real-time quantitative PCR (RT-qPCR) analysis, given the known interaction of the corresponding proteins with antifungals and with the efflux blocker FK506. The expression of the PDR1, MDR1, MDR2 and MDR4 genes was 2–4 times higher in the TRF-resistant strain grown in the presence of 0.14 µg/mL of TRF than in TRF-susceptible strains cultured in the absence of TRF. The TRF-resistant strain exhibited MICs of > 32 µg/mL for TRF alone; this resistance was attenuated to an MIC of 8 µg/mL in the presence of FK506, indicating that the TRF inhibitory concentration index value was < 0.75. The additive effect of the efflux blocker FK506 on TRF resistance was detected in the TRF-resistant strain. These results indicated that the TRF resistance in this strain reflects overexpression of genes encoding ABC transporter proteins.

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Fig. 1

Change history

  • 29 January 2018

    In the initial online publication, the name of author Hock Siew Han was given incorrectly as Han Hock Siew. The original article has been corrected.

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Acknowledgements

This study was supported by a grant (“International joint research and training of young researchers for zoonosis control in the globalized world”) from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan.

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Authors and Affiliations

  1. Department of Veterinary Pathobiology, Nihon University College of Bioresource Sciences, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan

    Rui Kano & Hiroshi Kamata

  2. Asian Veterinary Specialist Referral Center, 1F, No. 109, Sec. 4, XingYi Rd., Taipei, 106, Taiwan

    Yun-Hsia Hsiao & Charles Chen

  3. The Animal Clinic, Block 109, Clementi Street 11, #01-17/19, Singapore, 120109, Singapore

    Hock Siew Han

  4. Teikyo University Institute of Medical Mycology, 359 Otsuka, Hachioji, Tokyo, 192-0395, Japan

    Atsuhiko Hasegawa

Authors
  1. Rui Kano
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  2. Yun-Hsia Hsiao
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  3. Hock Siew Han
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  4. Charles Chen
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  5. Atsuhiko Hasegawa
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  6. Hiroshi Kamata
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Corresponding author

Correspondence to Rui Kano.

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The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this paper.

Additional information

The original version of this article was revised: In the initial online publication the name of author Hock Siew Han was given incorrectly as Han Hock Siew.

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Kano, R., Hsiao, YH., Han, H.S. et al. Resistance Mechanism in a Terbinafine-Resistant Strain of Microsporum canis. Mycopathologia 183, 623–627 (2018). https://doi.org/10.1007/s11046-018-0242-0

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  • DOI: https://doi.org/10.1007/s11046-018-0242-0

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