Skip to main content
SpringerLink
Log in

Development of Colletotrichum acutatum on Tolerant and Susceptible Olea europaea L. cultivars: A Microscopic Analysis

  • Published:
Mycopathologia Aims and scope Submit manuscript

Abstract

Colletotrichum acutatum is a cosmopolitan and damaging plant pathogen of temperate, subtropical, and tropical fruits and causes anthracnose on olive (Olea europaea L.). Three olive cultivars showing a variable response to infection by C. acutatum were selected to a preliminary study of pathogen development. Fruit samples, from susceptible and tolerant cultivars, were taken at 0, 24, 48, 72, and 192 h after inoculation for a microscopic and histological study of the infection and colonization process. The aim of this study was to compare the infection process: conidial germination, germ tube and appressorium formation, hyphal growth, and mesocarp colonization in susceptible and tolerant olive cultivars as a condition for further exploration of disease development, which is required to develop cultivars with improved resistance to anthracnose. The rate of mesocarp colonization differed between the susceptible and tolerant cultivars, and both intracellular hemibiotrophy and subcuticular intramural necrotrophy were observed. Hemibiotrophic infection predominated in the moderately tolerant cultivar.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Curry KI, Abril M, Avant JB, Smith BJ. Strawberry anthracnose: histopathology of Colletotrichum acutatum and C. fragariae. Phytopathology. 2002;92:1055–63. doi:10.1094/PHYTO.2002.92.10.1055.

    Article  PubMed  Google Scholar 

  2. Bailey JA, O’Connell RJ, Pring RJ, Nash C. Infection strategies of Colletotrichum species. In: Bailey JA, Jeger MJ, editors. Colletotrichum: biology pathology and control. Wallingford: CAB International; 1992. p. 88–120.

    Google Scholar 

  3. Wharton PS, Diéguez-Uribeondo J. The biology of Colletotrichum acutatum. Anales del Jardín Botánico de Madrid. 2004;61(1):3–22. doi:10.3989/ajbm.2004.v61.i1.61.

    Article  Google Scholar 

  4. Talhinhas P, Ferreira P, Neves-Martins J, Screenivasaprasad S, Oliveira H. Colletotrichum acutatum: principal agente causal da gafa da Oliveira em Portugal. III Simpósio Nacional de Olivicultura, Castelo-Branco, Portugal 2003.

  5. Sreenivasaprasad S, Talhinhas P. Genotypic and phenotypic diversity in Colletotrichum acutatum, a cosmopolitan pathogen causing anthracnose on a wide range of hosts. Mol Plant Pathol. 2005;6(4):361–78. doi:10.1111/j.1364-3703.2005.00291.x.

    Article  CAS  Google Scholar 

  6. Moldenhauer J, Moerschbacher BM, van der Westhuizen AJ. Histological investigation of stripe rust (Puccinia striiformis f. sp. tritici) development in resistant and susceptible wheat cultivars. Plant Pathol. 2006;55:469–74. doi:10.1111/j.1365-3059.2006.01385.x.

    Article  Google Scholar 

  7. Skipp RA, Beever RE, Sharrock KR, Rikkerink EHA, Templeton MD. Histopathological, genetic, biochemical and molecular basis in Colletotrichum. In: Singh US, Kohmoto K, Singh RP, editors. Pathogens and host parasite specificity in plant disease. Oxford: Elsevier Science Ltd; 1995. p. 119–43.

    Google Scholar 

  8. Perfect SE, Hughes HB, O’Connell RJ, Green J. Colletotrichum: a model genus for studies on pathology and fungal–plant interactions. Fungal Genet Biol. 1999;27:186–98. doi:10.1006/fgbi.1999.1143.

    Article  PubMed  CAS  Google Scholar 

  9. O’Connell RJ, Perfect SE, Hughes HB, Carazaniga R, Bailey JA. Dissecting the cell biology of Colletotrichum infection processes. In: Prusky D, Freeman S, Dickman MB, editors. Colletotrichum: host specificity, pathology, and host–pathogen interaction. St. Paul: The American Phytopathological Society; 2000. p. 57–76.

    Google Scholar 

  10. Politis DJ, Wheeler H. Ultrastructural study of penetration of maize leaves by Colletotrichum graminicola. Physiol Plant Pathol. 1973;3:465–71. doi:10.1016/0048-4059(73)90056-8.

    Article  Google Scholar 

  11. Ogle HJ, Gowanlock DH, Irwin JAG. Infection of Stylosanthes guianensis and S scabra by Colletotrichum gloeosporioides. Phytopathology. 1990;80:837–42. doi:10.1094/Phyto-80-837.

    Article  Google Scholar 

  12. Mercer PC, Wood RKS, Greenwood AD. Ultrastructure of parasitism of Phaseolus vulgaris by Colletotrichum lindemuthimum. Physiol Plant Pathol. 1975;5:203–14. doi:10.1016/0048-4059(75)90087-9.

    Article  Google Scholar 

  13. O’Connell RJ, Bailey JA, Richmond DV. Cytology and physiology of infection of Phaseolus vulgaris by Colletotrichum lindemuthianum. Physiol Mol Plant Pathol. 1985;27:75–98. doi:10.1016/0048-4059(85)90058-X.

    Article  Google Scholar 

  14. Arroyo FT, Moreno J, García-Herdugo G, De los Santos B, Barrau C, Porras M, et al. Ultrastructure of the early stages of Colletotrichum acutatum infection of strawberry tissues. Can J Bot. 2005;83(5):491–500. doi:10.1139/b05-022.

    Article  Google Scholar 

  15. Pring RJ, Nash C, Zakaria M, Bailey JA. Infection process and host range of Colletotrichum capsici. Physiol Mol Plant Pathol. 1995;46:137–52. doi:10.1006/pmpp.1995.1011.

    Article  Google Scholar 

  16. Shen S, Goodwin P, Hsiang T. Hemibiotrophic infection and identity of the fungus Colletotrichum destructivum, causing anthracnose of tobacco. Mycol Res. 2001;105:1340–7. doi:10.1017/S0953756201005111.

    Article  Google Scholar 

  17. Horowitz S, Freeman S, Sharon A. Use of green fluorescent protein-transgenic strains to study pathogenic and non-pathogenic lifestyles in Colletotrichum acutatum. Ecol Popul Biol. 2002;92(7):743–9.

    Google Scholar 

  18. Chongo G, Gossen BD, Bernier CC. Infection by Colletotrichum truncatum in resistant and susceptible lentil genotypes. Can J Plant Pathol. 2002;24:81–5.

    Google Scholar 

  19. Diéguez-Uribeondo J, Förster H, Soto-Estrada A, Adaskaveg JE. Subcuticular-intracellular hemibiotrophic and intracellular necrotrophic development of Colletotrichum acutatum on Almond. Phytopathology. 2005;95(7):751–8. doi:10.1094/PHYTO-95-0751.

    Article  PubMed  Google Scholar 

  20. Wharton PS, Schilder AC. Novel Infection strategies of Colletotrichum acutatum on ripe blueberry fruit. Plant Pathol. 2008;57:122–34.

    Google Scholar 

  21. Prieto P, Moore G, Shaw P. Fluorescence in situ hybridization on vibratome sections of plant tissues. Nat Protoc. 2007;2:1831–8. doi:10.1038/nprot.2007.265.

    Article  PubMed  CAS  Google Scholar 

  22. Kubo Y. Studies on mechanisms of appressorial penetration by Colletotrichum lagenarium. J Gen Plant Pathol. 2005;71:451–3. doi:10.1007/s10327-005-0229-9.

    Article  Google Scholar 

  23. Diéguez-Uribeondo J, Förster H, Adaskaveg JE. Digital image analysis of internal light spots of appressoria of Colletotrichum acutatum. Phytopathology. 2003;93(8):923–30. doi:10.1094/PHYTO.2003.93.8.923.

    Article  PubMed  Google Scholar 

  24. O’Connell RJ, Uronu AB, Waksman Nash GC, Keon JPR, Bailey JA. Hemibiotrophic infection of Pisum sativum by Colletotrichum truncatum. Plant Pathol. 1993;42:774–83. doi:10.1111/j.1365-3059.1993.tb01564.x.

    Article  Google Scholar 

  25. Smith JE, Korsten L, Aveling TAS. Infection process of Colletotrichum dematium on cowpea stems. Mycol Res. 1999;103(2):230–4. doi:10.1017/S0953756298006868.

    Article  Google Scholar 

  26. Bailey JA, Nash C, O’Connell RJ, Skipp RA. Infection process and host specificity of a Colletrotrichum species causing anthracnose disease of cowpea, Vigna unguiculata. Mycol Res. 1990;94:810–4. doi:10.1016/S0953-7562(09)81382-8.

    Article  Google Scholar 

  27. Zulfiqar M, Brlansky RH, Timmer LW. Infection of flower and vegetative tissues of citrus by Colletotrichum acutatum and C. gloeosporioides. Mycologia. 1996;88(1):121–8. doi:10.2307/3760791.

    Article  Google Scholar 

  28. Leandro LFS, Gleason ML, Nutter FW, Wegulo SN, Dixon PM. Germination and sporulation of Colletotrichum acutatum on symptomless strawberry leaves. Phytopathology. 2001;9:659–64. doi:10.1094/PHYTO.2001.91.7.659.

    Article  Google Scholar 

  29. Wharton PS, Schilder AMC. Infection and colonization of blueberry fruit by Colletotrichum acutatum. Phytopathology. 2003;93:S90.

    Google Scholar 

  30. Khan A, Hsiang T. The infection process of Colletotrichum graminicola and relative aggressiveness on four turfgrass species. Can J Microbiol. 2003;49:433–42. doi:10.1139/w03-059.

    Article  PubMed  CAS  Google Scholar 

  31. Latunde-Dada AO, Bailey JA, Lucas JA. Infection process of Colletotrichum destructivum O’Gara from Lucerne (Medicago sativa L.). Eur J Plant Pathol. 1997;103:35–41. doi:10.1023/A:1008698113368.

    Article  Google Scholar 

  32. Latunde-Dada AO, O’Connell RJ, Nash C, Pring RJ, Lucas JA, Bailey JA. Infection process and identity of the hemibiotrophic anthracnose fungus (Colletotrichum destructivum) from cowpea (Vigna unguiculata). Mycol Res. 1996;100:1133–41. doi:10.1016/S0953-7562(96)80226-7.

    Article  CAS  Google Scholar 

  33. Jeffries P, Dodd JC, Jeger MJ, Plumbery RA. The biology and control of Colletotrichum species on tropical fruit crops. Plant Pathol. 1990;39:343–66. doi:10.1111/j.1365-3059.1990.tb02512.x.

    Article  CAS  Google Scholar 

  34. Broekaert WF, Terras FRG, Cammue BPA. Induced and performed antimicrobial proteins. In: Slusarenko A, Fraser RSS, van Loon LC, editors. Mechanisms of resistance to plant disease. Dordrecht: Kluwer Academic; 2000. p. 371–477.

    Google Scholar 

  35. Mansfield JW. Antimicrobial compounds and resistance: the role of phytoalexins and phytoanticipins. In: Slusarenko A, Fraser RSS, van Loon LC, editors. Mechanisms of resistance to plant disease. Dordrecht: Kluwer Academic; 2000. p. 325–70.

    Google Scholar 

  36. Moldenhauer J, Moerschbacher BM, van der Westhuizen AJ. Histological investigation of stripe rust (Puccinia striiformis f. sp. tritici) development in resistant and susceptible wheat cultivars. Plant Pathol. 2006;55:469–74. doi:10.1111/j.1365-3059.2006.01385.x.

    Article  Google Scholar 

  37. Walker JC, Stahmann MA. Chemical nature of disease resistance in plants. Ann Rev Plant Physiol. 1955;6:351–64. doi:10.1146/annurev.pp.06.060155.002031.

    Article  CAS  Google Scholar 

  38. Matos LC, Andrade PereiraJA, PB SeabraRM, Beatriz M, Oliveira PP. Evaluation of a numerical method to predict the polyphenols content in monovarietal olive oils. Food Chem. 2007;102(3):976–83. doi:10.1016/j.foodchem.2006.04.026.

    Article  CAS  Google Scholar 

  39. Bentes JLS, Matsuoka K. Histologia da interacção Colletotrichum guaranicola e Paullinia cupana var. Sorbilis em clones resistente e suscetível. Fitolopatologa Brasileira. 2002;27:071–7.

    Google Scholar 

  40. Carvalho MT, Simões-Lopes P, Monteiro da Silva MJ, Pires S, Gonçalves MJ. The effect of Colletotrichum control on getting high quality olive oils. Proceedings of Second International Seminar: “Biotechnology and quality of olive tree products around the Mediterranean basin”, vol. II. 2006. p. 239–42.

  41. Kerstiens G. Plant cuticles: an integrated functional approach, ed. 1997. Oxford: BIOS Scientific Publishers; 1996. p. 337. ISBN 1 85996 130 4. Cambridge University Press Copyright © Cambridge University Press.

    Google Scholar 

Download references

Acknowledgments

This work was supported by Project ‘POCTI/AGR/57817/2004’ and a Ph.D. grant to Sónia Gomes, ‘SFRH/BD/25384/2005’ from the Fundação para a Ciência e Tecnologia, and by a ‘Proyecto de Excelencia- AGR649’ from the Junta de Andalucía.

Author information

Authors and Affiliations

  1. Institute of Biotechnology and Bioengineering, Centre of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro (IBB/CGB-UTAD), P.O. Box 1013, 5001-801, Vila Real, Portugal

    Sónia Gomes, Paula Martins-Lopes & Henrique Guedes-Pinto

  2. Department of Mejora Genética Vegetal, Instituto de Agricultura Sostenible (C.S.I.C.), Alameda del Obispo s/n., P.O. Box 4084, 14080, Cordoba, Spain

    Pilar Prieto & Antonio Martin

  3. National Plant Breeding Station, P.O. Box 6, 7350-951, Elvas, Portugal

    Teresa Carvalho

Authors
  1. Sónia Gomes
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pilar Prieto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Paula Martins-Lopes
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Teresa Carvalho
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Antonio Martin
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Henrique Guedes-Pinto
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sónia Gomes.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material (DOC 3597 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gomes, S., Prieto, P., Martins-Lopes, P. et al. Development of Colletotrichum acutatum on Tolerant and Susceptible Olea europaea L. cultivars: A Microscopic Analysis. Mycopathologia 168, 203–211 (2009). https://doi.org/10.1007/s11046-009-9211-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11046-009-9211-y

Keywords

Search

Navigation