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The effects and possible mechanism of action of apolipoprotein M on the growth of breast cancer cells

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Abstract

Background

To investigate the effects and mechanism of action of apolipoprotein M (ApoM) on the growth of breast cancer (BC) cells.

Methods and results

Bioinformatics, cell experiments and animal experiments were used to verify the effect of ApoM on breast cancer cell lines and breast tumor growth in vivo. ApoM expression was significantly reduced in BC tissues, and patients with lower ApoM mRNA expression had a poorer prognosis (P < 0.0001). Besides, ApoM can partially inhibit the proliferative, migratory and invasive processes of BC cells. In vivo, the difference between ApoM-OE and NC groups was no significant. The level of vitamin D receptor (VDR) protein in MDA-MB-231 cells was increased by overexpression of ApoM (P < 0.05), while in MCF-7 cells, VDR levels decreased (P < 0.05).

Conclusions

ApoM can partially inhibit the growth of BC cells. VDR may play a role, but is not the main pathway.

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Data availability

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Code availability

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Funding

This work was supported by the Changzhou International Scientific and Technological Cooperation Project (Grant No. CZ20180018) and the Major Science and Technology Projects of Changzhou Health and Family Planning Commission (Grant No. ZD201803).

Author information

Authors and Affiliations

  1. Comprehensive Laboratory, The Third Affiliated Hospital of Soochow University, 185 Juqiang St, Changzhou, Jiangsu Province, China

    Ying Zhou, Shuang Yao, Miaomei Yu, Jiang Wei & Guanghua Luo

  2. Clinical Medical Research Center, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu Province, China

    Ying Zhou, Shuang Yao, Miaomei Yu, Jiang Wei & Guanghua Luo

  3. Breast Surgery, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu Province, China

    Qi Fang

  4. Division of Clinical Chemistry and Pharmacology, Department of Laboratory Medicine, Lunds University, 22185, Lund, Sweden

    Ning Xu

Authors
  1. Ying Zhou
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  4. Jiang Wei
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  7. Guanghua Luo
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Contributions

GL, NX and MY contributed to the study conception and design. Material preparation, data collection and analysis were performed by YZ and SY. The first draft of the manuscript was written by YZ and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Guanghua Luo.

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The authors have no relevant financial or non-financial interests to disclose.

Ethical approval

The experimental protocol was established, according to the ethical guidelines of the Helsinki Declaration and was approved by the Human Ethics Committee of the third affiliated hospital of Soochow University.

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Participants provided written informed consent before enrolment.

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Zhou, Y., Yao, S., Yu, M. et al. The effects and possible mechanism of action of apolipoprotein M on the growth of breast cancer cells. Mol Biol Rep 49, 1171–1179 (2022). https://doi.org/10.1007/s11033-021-06945-2

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  • DOI: https://doi.org/10.1007/s11033-021-06945-2

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