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ATP-binding cassette transporters expression profiling revealed its role in the development and regulating stress response in Solanum tuberosum

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Abstract

The ATP-binding cassette (ABC) transporter gene family plays a vital role in substance transportation, including secondary metabolites, and phytohormones across membranous structures. It is still uncovered in potato (Solanum tuberosum), grown worldwide as a 3rd important food crop. The current study identified a total of 54 Stabc genes in potato genome. The accumulative phylogenetic tree of Stabc with arabidopsis, divided into eight groups (ABCA to ABCH). ABCG was the most prominent group covering 90% of Stabc genes, followed by ABCB group. The number and architecture of exon–intron varied from gene to gene. In addition, the presence of stress-responsive elements in the regulatory regions depicted their role in environmental stress. Furthermore, the tissue-specific and stress-specific expression profiling of Stabc genes and their validation through real-time-qPCR analysis revealed their role in development and stress. The presented results provided useful information for further functional analysis of Stabc genes and can also use as a reference study for other important crops.

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Funding

This work was supported by Shenzhen Sustainable Development Science and Technology Project (KCXFZ20201221173404012, KCXFZ20201221173211033) and and International Foundation for Science (IFS: C-6501-1).The authors appreciate the support of the Research Center for Advanced Materials Science (RCAMS) at King Khalid University Abha, Saudi Arabia through a Grant KKU/RCAMS/G002-21.

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Author notes

  1. Madiha Zaynab and Zongkang Wang are co-first authors.

Authors and Affiliations

  1. Shenzhen Key Laboratory of Marine Bioresource & Eco-Environmental Sciences, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 51807, Guangdong, China

    Madiha Zaynab & Shuangfei Li

  2. Shenzhen Batian Ecotypic Engineering Company Limited, Shenzhen, 518105, China

    Zongkang Wang

  3. Genomics Lab, Department of Life Science, University of Management and Technology (UMT), Lahore, 54770, Pakistan

    Athar Hussain

  4. National Agriculture Research Center, PARC Institute of Advanced Studies in Agriculture, Islamabad, Pakistan

    Khalida Bahadar

  5. Department of Horticulture, Ghazi University, Dera Ghazi Khan, 32200, Pakistan

    Mateen Sajid

  6. College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China

    Yasir Sharif

  7. Institute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan

    Muhammad Azam

  8. Department of Biochemistry & Biotechnology, Hafiz Hayat Campus University of Gujrat, Gujrat City, Pakistan

    Kalsoom Sughra

  9. Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, China

    Muhammad Ammar Raza

  10. Research Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha, 61413, Saudi Arabia

    Khalid Ali Khan

  11. Unit of Bee Research and Honey Production, Faculty of Science, King Khalid University, P.O. Box 9004, Abha, 61413, Saudi Arabia

    Khalid Ali Khan

  12. Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha, 61413, Saudi Arabia

    Khalid Ali Khan

Authors
  1. Madiha Zaynab
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  2. Zongkang Wang
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  3. Athar Hussain
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  4. Khalida Bahadar
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  5. Mateen Sajid
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  6. Yasir Sharif
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  7. Muhammad Azam
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  8. Kalsoom Sughra
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  9. Muhammad Ammar Raza
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  10. Khalid Ali Khan
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  11. Shuangfei Li
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Contributions

MZ designed the study. MZ, AH, KB and YS conducted the experiments and analyzed the data. KS, MS, MAR, XY, KAK contributed to writing and editing the manuscript. ZW, SL supervised the project. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Shuangfei Li.

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Zaynab, M., Wang, Z., Hussain, A. et al. ATP-binding cassette transporters expression profiling revealed its role in the development and regulating stress response in Solanum tuberosum. Mol Biol Rep 49, 5251–5264 (2022). https://doi.org/10.1007/s11033-021-06697-z

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