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Effects of miR-125b-5p on Preadipocyte Proliferation and Differentiation in Chicken

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Abstract

Our previous studies have shown that miR-125b-5p was highly expressed and significantly upregulated during abdominal fat deposition in chickens. However, the role of miR-125b in the regulation of adipogenesis is not clear in chickens. Therefore, we evaluated the effects of miR-125b-5p on preadipocyte proliferation and differentiation and the interaction between miR-125b-5p and the acyl-CoA synthetase bubblegum family member 2 (ACSBG2) gene in adipogenesis in chicken abdominal adipose tissue. Here, transfection tests of miR-125b-5p mimic/inhibitor were performed in preadipocytes, and the effects of miR-125b-5p on preadipocytes proliferation and differentiation were analyzed. The target site of miR-125b-5p in the 3′UTR (untranslated region) of ACSBG2 were verified by a luciferase reporter assay. Our results showed that miR-125b-5p overexpression inhibited proliferation and reduced the number of cells in S phase and G2/M phase in preadipocytes; conversely, miR-125b-5p inhibition promoted the proliferation and increased the number of cells in S phase and G2/M phase. In adipocytes after induction, miR-125b-5p overexpression led to a notable increase in the accumulation of lipid droplets as well as in the concentration of triglycerides, while miR-125b-5p inhibition had the opposite effect. Furthermore, miR-125b-5p could directly bind to the 3'UTR of ACSBG2, and its overexpression could significantly repress the mRNA and protein expression of ACSBG2. These results indicate that miR-125b-5p can inhibit preadipocyte proliferation and can promote preadipocyte differentiation to affect adipogenesis in chicken abdominal adipose tissues, at least partially by downregulating ACSBG2.

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Data Availability

The data and material used and analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This study was funded by a grant from the National Natural Science Foundation of China (Nos. 32072692 and 31572356), the Program for Innovation Research Team of Ministry of Education (IRT16R23) and the Scientific Studio of Zhongyuan Scholars (No. 30601985).

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  1. Guoxi Li and Yi Chen contributed equally to this work.

Authors and Affiliations

  1. College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan Province, 450002, P. R. China

    Guoxi Li, Yi Chen, Wenjiao Jin, Bin Zhai, Yuanfang Li, Guirong Sun, Hong Li, Xiangtao Kang & Yadong Tian

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  1. Guoxi Li
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  2. Yi Chen
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  3. Wenjiao Jin
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Contributions

Conceptualization, G.L.; data curation, Y.L. and G.S.; formal analysis, W.J.; funding acquisition, G.L., Y.T., and X.K.; investigation, G.L., Y.C., W.J., and B.Z.; resources, H.L. and X.K.; software, Z.L. and W.L.; validation, Y.Z. and B.Z.; visualization, Y.C.; writing—original draft, S.F.; writing—review and editing, G.L. and Y.C.

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Correspondence to Guoxi Li or Yadong Tian.

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Li, G., Chen, Y., Jin, W. et al. Effects of miR-125b-5p on Preadipocyte Proliferation and Differentiation in Chicken. Mol Biol Rep 48, 491–502 (2021). https://doi.org/10.1007/s11033-020-06080-4

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