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Isolation and characterization of 20 polymorphic microsatellites loci for Xenocypris davidi based on high-throughput sequencing

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Abstract

Xenocypris davidi is one of the most economically important freshwater fish in China. However, few molecular markers have been reported for this species, impeding in-depth population genetic, dispersal, and gene flow studies. In the present study, a batch of novel polymorphic microsatellites from the genome of X. davidi were isolated and characterized using high-throughput sequencing. A total of 20 microsatellite markers were isolated. Analysis of 33 individuals revealed an average of 7.35 alleles per locus, ranging from 3 to 18. The observed and expected heterozygosities ranged from 0.3 to 1 and from 0.426 to 0.93, respectively. Only one tested locus significantly deviated from Hardy–Weinberg equilibrium. 18 microsatellite loci were highly polymorphic (PIC > 0.5). These newly isolated microsatellite markers would be useful to study the population genetics and stock management of X. davidi.

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Acknowledgments

Our experimental procedures complied with the current laws on animal welfare and research in China. The study was financed by the public welfare project of Zhejiang Province (LGN18C190001). We also thank the anonymous reviewers for their improvements in the manuscript.

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Authors and Affiliations

  1. Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture, Key Laboratory of Freshwater Aquaculture Genetic and Breeding of Zhejiang Province, Zhejiang Research Center of East China Sea Fishery Research Institute, Zhejiang Institute of Freshwater Fisheries, 999th South Hangchangqiao Road, Wuxing District, Huzhou, 313000, China

    Aihuan Guo, Julin Yuan, Qingping Lian & Zhimin Gu

  2. Jinhua Fisheries Technology Extension Station, Jinhua, 321000, China

    Ming Li

Authors
  1. Aihuan Guo
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  2. Julin Yuan
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  3. Qingping Lian
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  4. Ming Li
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  5. Zhimin Gu
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Corresponding authors

Correspondence to Julin Yuan or Zhimin Gu.

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The authors declare no competing interests.

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All the procedures in this study were approved and carried out in accordance with the laboratory animal care protocol from the Animal welfare committee of Zhejiang institute of freshwater fisheries.

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Accession codes

Sequences containing the microsatellite loci were deposited in GenBank under Accession Numbers MK888833–MK888852.

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Guo, A., Yuan, J., Lian, Q. et al. Isolation and characterization of 20 polymorphic microsatellites loci for Xenocypris davidi based on high-throughput sequencing. Mol Biol Rep 47, 8305–8310 (2020). https://doi.org/10.1007/s11033-020-05834-4

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  • DOI: https://doi.org/10.1007/s11033-020-05834-4

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