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Developing novel microsatellite markers by NGS technology for Rhopilema nomadica, an invasive jellyfish

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Abstract

Twelve microsatellite loci, obtained by whole genome sequencing approach, were developed and validated for the rhizostomatid jellyfish Rhopilema nomadica, the most pernicious invasive species in the Mediterranean Sea. A sample of 40 specimens collected at six locations along the Mediterranean coast of Israel were genotyped and all loci presented suitable outcomes to population genetic studies, revealing 5–19 alleles/locus with clean and reproducible amplifications. Observed and expected heterozygosity ranged 0.0.353 to 0.971 and 0.335 to 0.870, respectively, and the fixation index (inbreeding coefficient) and the polymorphic information content (PIC) ranged between  − 0.190 and 0.240 and 0.32 to 0.858, respectively. The new set of microsatellite loci will be used to study long-term changes in the population genetic parameters of this invasive species.

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Acknowledgements

Thanks to O. Klein for his photograph of a Rhopilema swarm. We thank Rinkevich’s and Lubinevsky’s labs members for the help in jellyfish collections. This study was supported by the Israeli Ministry of Energy, contract Nos.: 211–17-023 and-218–17-009

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Authors and Affiliations

  1. Israel Oceanographic and Limnological Research, National Institute of Oceanography, 31080, Haifa, Israel

    Jacob Douek, Zoya Harbuzov & Baruch Rinkevich

  2. Steinhardt Museum of Natural History, Tel Aviv University, 69978, Tel Aviv, Israel

    Bella S. Galil

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  1. Jacob Douek
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  2. Zoya Harbuzov
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  3. Bella S. Galil
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  4. Baruch Rinkevich
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Correspondence to Jacob Douek.

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Douek, J., Harbuzov, Z., Galil, B.S. et al. Developing novel microsatellite markers by NGS technology for Rhopilema nomadica, an invasive jellyfish. Mol Biol Rep 47, 4821–4825 (2020). https://doi.org/10.1007/s11033-020-05533-0

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