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Anticancer and antimetastatic activity of Hypomyces chrysospermus, a cosmopolitan parasite in different human cancer cells

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Abstract

The importance of microbial natural compounds in drug research is increasing every year and they are used to prevent or treat a variety of diseases. Hypomyces chrysospermus is a cosmopolitan parasite of many Boletaceae members. Since not much work has been conducted to date, this study is undertaken to explore the anticancer effect, including the antiproliferative and antimetastatic activity of Hypomyces chrysospermus. The aim of this study is to determine the antiproliferative and antimetastatic activity of Hypomyces chrysospermus ethyl acetate extract, having antioxidant activity, against A549, Caco2, MCF-7 human cancer and CCD-19 Lu and CCD 841 CoN healthy human cell lines. Firstly, cytotoxic activity was determined by the WST-1 assay. After cell proliferations and anti-metastatic effects were investigated by a real-time cell analysis system (RTCA-DP) and IC50 concentrations were calculated for each cell line. In addition, the expression levels of Apaf-1, TNF and NF-kB mRNA in cancer cells were investigated with RealTime-PCR. The ethyl acetate extract of Hypomyces chrysospermus presented anticancer activities including antiproliferative and antimetastatic effects. Hypomyces chrysospermus as a source of biologically active metabolites can be used as an important resource in the development of new anticancer effective agents.

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Acknowledgements

This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) under Grant No. 113Z746. The authors extend their thanks to Dr. Bükay Yenice Gürsu for technical contribution of the dendrogram.

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Authors and Affiliations

  1. Faculty of Pharmacy, Department of Pharmacology, Anadolu University, 26210, Eskisehir, Turkey

    Miriş Dikmen

  2. Tavas Vocational School of Health Services, Pamukkale University, Denizli, Turkey

    Selin Engür Öztürk

  3. Faculty of Pharmacy, Department of Pharmaceutical Microbiology, Anadolu University, 26210, Eskisehir, Turkey

    Zerrin Cantürk

  4. Public Health Research and Application Center, Central Immunology Laboratory, Gazi University, Ankara, Turkey

    Göksu Ceylan

  5. Duzen Norwest Laboratories, Kaptanpasa Street, No: 2, Buyukesat District, 06700, Ankara, Turkey

    Ayşe Betül Karaduman

  6. Faculty of Science and Letters, Department of Biology, Eskisehir Osmangazi University, 26480, Eskisehir, Turkey

    Mustafa Yamaç

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  1. Miriş Dikmen
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  2. Selin Engür Öztürk
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Correspondence to Miriş Dikmen.

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There is no conflict of interest in this study.

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This work was carried out on cell lines (A549, Caco2, MCF-7, CCD-19 Lu and CCD 841 CoN), with no requirement for ethical clearance.

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Dikmen, M., Öztürk, S.E., Cantürk, Z. et al. Anticancer and antimetastatic activity of Hypomyces chrysospermus, a cosmopolitan parasite in different human cancer cells. Mol Biol Rep 47, 3765–3778 (2020). https://doi.org/10.1007/s11033-020-05468-6

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  • DOI: https://doi.org/10.1007/s11033-020-05468-6

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