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Genome-wide association mapping for adult resistance to powdery mildew in common wheat

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Abstract

Blumeria graminis f. sp. tritici, the causal agent of wheat powdery mildew disease, can occur at all stages of the crop and constantly threatens wheat production. To identify candidate resistance genes for powdery mildew, we performed GWAS (genome-wide association studies) on a total set of 329 wheat varieties obtained from different origins. These wheat materials were genotyped using wheat 90K SNP array and evaluated for their resistance in either field or glasshouse condition from 2016 to 2018. Using a mixed linear model, 33 SNP markers of which 14 QTL (quantitative trait loci) were later defined were observed to associate with powdery mildew resistance. Among these, QTL on chromosome 3A, 3B, 6D and 7D were concluded as potentially new QTL. Exploration of candidate genes for new QTL suggested roles of these genes involved in encoding disease resistance and defence-related proteins, and regulating early immune response to the pathogen. Overall, the results reveal that GWAS can be an effective means of identifying marker-trait associations, though further functional validation and fine-mapping of gene candidates are required before creating opportunities for developing new resistant genotypes.

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Acknowledgements

We would like to thank Dr. Ross Corkrey for providing valuable suggestions on statistical analysis.

Funding

This work was supported by the Grains Research and Development Corporation of Australia, Grant UT00030 and the Fundamental Research Funds for the Central Universities (2019QNA6022).

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Authors and Affiliations

  1. TIA, University of Tasmania, Private Bag 1375, Launceston, TAS, 7250, Australia

    Yichen Kang & Meixue Zhou

  2. TIA, University of Tasmania, Private Bag 98, Hobart, TAS, 7001, Australia

    Karen Barry

  3. Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, 310058, China

    Fangbing Cao

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  1. Yichen Kang
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  2. Karen Barry
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  3. Fangbing Cao
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  4. Meixue Zhou
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Contributions

YK conducted the phenotyping for the 2017 and 2108 studies performed the GWAS analysis and drafted the manuscript. FC conducted the phenotyping for the 2016 study. KB contributed to editing the manuscript. MZ conceived the study, generated the genotyping data and critically reviewed the mauscript.

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Correspondence to Meixue Zhou.

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Kang, Y., Barry, K., Cao, F. et al. Genome-wide association mapping for adult resistance to powdery mildew in common wheat. Mol Biol Rep 47, 1241–1256 (2020). https://doi.org/10.1007/s11033-019-05225-4

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  • DOI: https://doi.org/10.1007/s11033-019-05225-4

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