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Development of 12 microsatellite markers for Bombina orientails based on RNA-Seq and their usefulness in population genetic diversity

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Abstract

Bombina orientalis is widely used due to bombesin which isolated from its skin. But in recent years, the population of B. orientalis has become declining distinctly because of human activities, environmental pollution, drought climatic conditions and other factors. In order to provide the molecular basis for the proposal of biodiversity conservation, we report the development of 12 microsatellite markers for B. orientalis based on RNA-Seq. We test polymorphism against in 48 B. orientalis individuals which randomly selected from 182 individuals take advantage of polyacrylamide gel electrophoresis (PAGE). These markers will be useful in the research on the genetic diversity, population genetic structure and other studies. For B. orientalis, all of these loci showed polymorphism, and in line with the H–W equilibrium law. The number of alleles per locus ranged from 3 to 21. The observed and expected heterozygosities ranged from 0.0118 to 0.7795 and from 0.1612 to 0.8703, respectively. The polymorphism information content ranged from 0.153 to 0.857. And the genetic diversity of B. orientalis in Lushui Rivers is significantly higher than that in the Maoer Mountains.

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Funding

This work was supported by the Natural Science Foundation of Heilongjiang Province (#QC2013C016 to CX).

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Authors and Affiliations

  1. College of Life Science, Northeast Agricultural University, No. 600 Changjiang Road, Xiangfang District, Harbin, 150030, China

    Yanshuang Shi, Liqun Yu, Xiaomin Han, Shuai Zhao, Tianfu Niu & Chunzhu Xu

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  1. Yanshuang Shi
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  2. Liqun Yu
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  3. Xiaomin Han
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  4. Shuai Zhao
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  5. Tianfu Niu
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  6. Chunzhu Xu
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Contributions

YS conducted most of the data analysis and drafted the manuscript. LY, XH, SZ, TN carried out the development of microsatellite markers experiments. CX conceived the project and finalized the manuscript. All authors participated in its design, read, and approved the final manuscript.

Corresponding author

Correspondence to Chunzhu Xu.

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The author declare that they have no conflict of interest.

Ethical approval

This study does not involve any species at risk of extinction. This study was conducted with ethical approval by the Institutional Animal Care and Use Committee (IACUC) of Northeast Agricultural University (Harbin, China) according to the national and international guidelines.

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Shi, Y., Yu, L., Han, X. et al. Development of 12 microsatellite markers for Bombina orientails based on RNA-Seq and their usefulness in population genetic diversity. Mol Biol Rep 45, 2811–2814 (2018). https://doi.org/10.1007/s11033-018-4371-9

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  • DOI: https://doi.org/10.1007/s11033-018-4371-9

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