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Polymorphism distribution of RYR1, PRKAG3, HFABP, MYF-5 and MC4R genes in crossbred pigs

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Abstract

This study was designed to screen the crossbred pigs for SNPs in five candidate genes, associated with pork quality traits and to differentiate their genotypes by PCR–RFLP. The results indicated that genotypes of crossbred pigs were NN (90%) and Nn (10%) for RYR1; RR (83%) and QR (17%) for PRKAG3; HH (98%), Hh (1%) and hh (1%) for HFABP; DD (99%) and CD (1%) for MYF-5; and AG (57%), GG (26%) and AA (17%) for MC4R SNPs, respectively. Allelic frequencies for five SNPs {RYR1 (1843C>T), PRKAG3 (c.599G>A), HFABP (c.1322C>T), MYF-5 (c.1205A>C) and MC4R (c.1426A>G)} were 0.95 and 0.05 (N/n), 0.08 and 0.92 (Q/R), 0.99 and 0.01 (H/h), 0.00 and 1.00 (C/D) and 0.45 and 0.55 (A/G), respectively. The effect of RYR1 (1843C>T) SNP was significant on pH45 (P < 0.05), pH24 (P < 0.05) and protein % (P < 0.05). The PRKAG3 (c.599G>A) and MC4R (c.1426A>G) SNP had significant association with dressing percentages. The results revealed that RYR1, PRKAG3 and MC4R SNPs may be used in marker associated selection for pork quality traits in crossbred pigs.

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Acknowledgements

The help rendered by Director, Joint Director (Research) and Joint Director (Academic); Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh and financial support by Indian Council of Agricultural Research, New Delhi is highly acknowledged.

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Authors and Affiliations

  1. Division of Animal Genetics, ICAR-IVRI, Izatnagar, 243122, India

    B. L. Saini, N. R. Sahoo, A. Kumar, B. C. Naha & A. Baranwal

  2. Livestock Production and Management Section, ICAR-IVRI, Izatnagar, 243122, India

    G. K. Gaur

  3. Division of Livestock Products Technology, ICAR-IVRI, Izatnagar, 243122, India

    S. K. Mendiratta & R. K. Jaiswal

  4. Dairy Cattle Breeding Division, ICAR-NDRI, Karnal, 132001, India

    V. Yadav

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  1. B. L. Saini
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Saini, B.L., Gaur, G.K., Sahoo, N.R. et al. Polymorphism distribution of RYR1, PRKAG3, HFABP, MYF-5 and MC4R genes in crossbred pigs. Mol Biol Rep 45, 1575–1585 (2018). https://doi.org/10.1007/s11033-018-4263-z

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