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Protective action of curcumin and nano-curcumin against arsenic-induced genotoxicity in rats in vivo

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Abstract

We explored whether nanoformulation of curcumin can cause better protective effect than free curcumin against arsenic-induced genotoxicity. Curcumin-loaded Poly(lactic-co-glycolic acid) nanoparticles (CUR-NP) were prepared by emulsion technique. The CUR-NP were water soluble and showed biphasic release pattern. Rats were divided into 5 groups of 6 each. Group I served as the control. Group II rats were exposed to sodium arsenite (25 ppm) daily through drinking water for 42 days. Groups III, IV and V were maintained as in Group II, however, they were also administered empty nanoparticle, curcumin (100 mg/kg bw) and CUR-NP (100 mg/kg bw), respectively, by oral gavage during the last 14 days of arsenic exposure. On the 43rd day, genotoxic effects were evaluated in bone marrow cells. Arsenic increased chromosomal aberrations, micronuclei formation and DNA damage. Both free curcumin and CUR-NP attenuated these arsenic-mediated genotoxic effects. However, the result suggests that nanoformulation have better protective effect than free curcumin at the same dose level.

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Abbreviations

CUR-NP:

Curcumin-loaded Poly(lactic-co-glycolic acid) nanoparticles

PLGA:

Poly(lactic-co-glycolic acid)

PVA:

Polyvinyl alcohol

PBS:

Phosphate buffered saline

eNPs:

Empty nanoparticles

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Acknowledgments

The authors are thankful to the Department of Anatomy, Govind Ballabh Pant University of Agriculture and Technology (GBPUAT), Pantnagar, Uttarakhand, India, for Transmission Electron Microscopic analysis of CUR-NP. The authors are grateful to the Director, Indian Veterinary Research Institute, for providing necessary facilities.

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  1. Palanisamy Sankar

    Present address: Department of Veterinary Pharmacology and Toxicology, Veterinary College and Research Institute, Orathanadu, 614625, Tamil Nadu, India

Authors and Affiliations

  1. Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, Bareilly, 243 122, Uttar Pradesh, India

    Avinash Gopal Telang, Karunakaran Vijayakaran, Manickam Kesavan & Souvendra Nath Sarkar

  2. Department of Veterinary Microbiology, Veterinary College and Research Institute, Namakkal, 637002, Tamil Nadu, India

    Kalaivanan Ramya

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  1. Palanisamy Sankar
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Correspondence to Palanisamy Sankar.

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Sankar, P., Telang, A.G., Ramya, K. et al. Protective action of curcumin and nano-curcumin against arsenic-induced genotoxicity in rats in vivo. Mol Biol Rep 41, 7413–7422 (2014). https://doi.org/10.1007/s11033-014-3629-0

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