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In vitro studies on the interaction between human serum albumin and fosfomycin disodium salt, an antibiotic drug by multi-spectroscopic and molecular docking methods

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Abstract

The interaction between the human serum albumin (HSA) and drug, fosfomycin disodium salt (FOS) has been studied by different spectroscopic techniques. The experimental results showed a static quenching mechanism in the interaction of FOS with HSA. The number of binding sites, n and observed binding constant K a were measured by fluorescence quenching method. The thermodynamic parameters ΔG°, ΔH° and ΔS° were calculated according to van’t Hoff equation. The calculated distance r between FOS and the protein is evaluated according to the theory of Förster energy transfer. A change in the secondary structure of the protein was evident from the circular dichroism measurements, synchronous fluorescence and three-dimensional fluorescence spectra.

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Acknowledgments

One of the author Manjunath D. Meti thanks DST New Delhi for providing Innovation in Science Pursuit for Inspired Research (INSPIRE) fellowship. The authors thank the Prof. M. V. Kulkarni, Department of Chemistry, K. U. Dharwad for valuable suggestions. The authors also thank the Chairman, Department of Molecular Biophysics, Indian Institute of Science, Bangalore for CD measurement facilities and Dr. V. H. Kulkarni, Principal, S.E.T’s College of Pharmacy, Dharwad for docking studies.

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Authors and Affiliations

  1. P. G. Department of Studies in Chemistry, Karnatak University, Pavate Nagar, Dharwad, 580003, India

    Manjunath D. Meti, Kirthi S. Byadagi, Sharanappa T. Nandibewoor & Shivamurti A. Chimatadar

  2. Novel Drug Design and Discovery Laboratory, Department of Pharmaceutical Chemistry, S.E.T’s College of Pharmacy, Sangolli Rayanna Nagar, Dharwad, 580002, Karnataka, India

    Shrinivas D. Joshi & Uttam A. More

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  1. Manjunath D. Meti
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Correspondence to Shivamurti A. Chimatadar.

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Meti, M.D., Byadagi, K.S., Nandibewoor, S.T. et al. In vitro studies on the interaction between human serum albumin and fosfomycin disodium salt, an antibiotic drug by multi-spectroscopic and molecular docking methods. Mol Biol Rep 41, 2377–2387 (2014). https://doi.org/10.1007/s11033-014-3092-y

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  • DOI: https://doi.org/10.1007/s11033-014-3092-y

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