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Detection of interaction between lysionotin and bovine serum albumin using spectroscopic techniques combined with molecular modeling

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Abstract

A combination of fluorescence, UV–Vis absorption, circular dichroism (CD), Fourier transform infrared (FT-IR) and molecular modeling approaches were employed to determine the interaction between lysionotin and bovine serum albumin (BSA) at physiological pH. The fluorescence titration suggested that the fluorescence quenching of BSA by lysionotin was a static procedure. The binding constant at 298 K was in the order of 105 L mol−1, indicating that a high affinity existed between lysionotin and BSA. The thermodynamic parameters obtained at different temperatures (292, 298, 304 and 310 K) showed that the binding process was primarily driven by hydrogen bond and van der Waals forces, as the values of the enthalpy change (ΔH°) and entropy change (ΔS°) were found to be −40.81 ± 0.08 kJ mol−1 and −35.93 ± 0.27 J mol−1 K−1, respectively. The surface hydrophobicity of BSA increased upon interaction with lysionotin. The site markers competitive experiments revealed that the binding site of lysionotin was in the sub-domain IIA (site I) of BSA. Furthermore, the molecular docking results corroborated the binding site and clarified the specific binding mode. The results of UV–Vis absorption, CD and FT-IR spectra demonstrated that the secondary structure of BSA was altered in the presence of lysionotin.

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Acknowledgments

We gratefully acknowledge the financial support of the National Natural Science Foundation of China (Nos. 21167013 and 31060210), the Natural Science Foundation of Jiangxi Province (20114BAB204019), and the Research Program of State Key Laboratory of Food Science and Technology of Nanchang University (Nos. SKLF-ZZB-201305, SKLF-ZZA-201302 and SKLF-KF-201203).

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  1. State Key Laboratory of Food Science and Technology, Nanchang University, No. 235, Nanjing East Road, Nanchang, 330047, Jiangxi, China

    Yuting Hu, Guowen Zhang & Jiakai Yan

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  1. Yuting Hu
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  2. Guowen Zhang
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  3. Jiakai Yan
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Correspondence to Guowen Zhang.

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Hu, Y., Zhang, G. & Yan, J. Detection of interaction between lysionotin and bovine serum albumin using spectroscopic techniques combined with molecular modeling. Mol Biol Rep 41, 1693–1702 (2014). https://doi.org/10.1007/s11033-013-3018-0

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  • DOI: https://doi.org/10.1007/s11033-013-3018-0

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