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Lack of association between cyclooxygenase 2−765G/C gene polymorphism and breast cancer risk in Ahvaz, west-south Iran

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Abstract

Cyclooxygenases are key enzymes in conversion of arachidonic acid into prostaglandin H2. Cyclooxygenase-2 (COX-2) increases prostaglandins in neoplastic tissue. COX-2 has important roles in cell proliferation cancers, angiogenesis, and alzheimer. COX-2 is up-regulated in several types of cancer, and it is hypothesized that COX-2 expression may be genetically influenced. Our main objective was to evaluated the association of polymorphism COX-2 with risk of breast cancer in khouzestan province, and the second objective of the study was to evaluate the association with biochemistry parameters. This study consisting of 150 patients with breast cancer and 120 normal DNA was extracted from the white blood cells. Polymorphism cox2 gene was detected by polymerase chain reaction according to the standard methods. The profile lipids and estrogen were measured in two groups by standard methods. Chi square analysis showed that there was no association between breast cancer risk and COX-2 −765G>C genotype and alleles. Also, no association were observed between −765G>C polymorphism and biochemistry parameters. A multiple logistic regression model with cox2 genotypes and LDL and HDL as covariates revealed that there is no significant association between cox2 genotypes and risk of breast cancer, but higher values of LDL and HDL significantly increase risk of breast cancer.

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Acknowledgments

The authors thank all the subjects for participating. This study was supported by the Department of Biochemistry, Faculty of Medicine, Ahvaz Jondishapour University of Medical sciences, Ahvaz, Iran.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medicine, Ahvaz Jondishapour University of Medical Sciences, Ahvaz, Iran

    Behnaz Ahmadi, Abdolrahim Nikzamir, Seid Mohamadali Ghafari, Ghorban Mohamadzadeh & Ahmad Bafandeh

  2. Endocrine Research Center, Valiasr Hospital, Tehran University of Medical Sciences, Tehran, Iran

    Abdolrahim Nikzamir

  3. Health Department, Faculty of Health, Ahvaz Jondishapour University of Medical Sciences, Ahvaz, Iran

    Mahmod Latifi

  4. Anesthesiology Department, Faculty of Medicine, Mofid Children’s Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Mohammad Fathi

  5. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

    Mir Saeed Yekaninejad

  6. Farzanegan 2 High School, National Organization of Developing Exceptional Talents, Tehran, Iran

    Mahfam Nikzamir

Authors
  1. Behnaz Ahmadi
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  2. Abdolrahim Nikzamir
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  3. Seid Mohamadali Ghafari
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  4. Ghorban Mohamadzadeh
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  6. Ahmad Bafandeh
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  7. Mohammad Fathi
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  8. Mir Saeed Yekaninejad
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  9. Mahfam Nikzamir
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Correspondence to Abdolrahim Nikzamir or Mohammad Fathi.

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Ahmadi, B., Nikzamir, A., Ghafari, S.M. et al. Lack of association between cyclooxygenase 2−765G/C gene polymorphism and breast cancer risk in Ahvaz, west-south Iran. Mol Biol Rep 41, 997–1001 (2014). https://doi.org/10.1007/s11033-013-2944-1

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  • DOI: https://doi.org/10.1007/s11033-013-2944-1

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