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Sequence analysis of bovine C/EBPδ gene and its adipogenic effects on fibroblasts

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Abstract

CCAAT/enhancer binding protein delta (C/EBPδ), an important transcriptional factor, regulates cell growth, differentiation and adipogenesis in humans and mice. However, we lack of directive information on the effects of C/EBPδ gene in bovine cells. In the present study, we cloned the CDS areas of bovine C/EBPδ gene and predicted its sequence characteristics. Moreover, we constructed the recombinant adenovirus plasmids of bovine C/EBPδ gene and harvested the subsequent adenoviruses to infect bovine primary fibroblasts. Oil Red O staining results showed lipid droplets accumulated gradually in the adenoviruses treated fibroblasts. Time course real-time PCR results indicated that over-expression of exogenous C/EBPδ regulated the mRNA expression levels of some key adipogenic genes, herein, activated the C/EBPα expression, increased lipoprotein lipase and fatty acid binding protein 4 mRNA expression levels, whereas inhibited leptin receptor gene. In conclusion, the present study demonstrates that the elevated C/EBPδ can induce the adipogenesis in the fibroblasts of cattle.

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Acknowledgments

Research supported by the China National Twelfth “Five Year” Science and Technology Support Project (2011BAD28B04-03), China National 863 Program (2011AA100307), China GMO New Varieties Major Project (2011ZX08007-002), China National Beef and Yak Industrial Technology System (CARS-38), Program for Changjiang Scholars and Innovative Research Team of China (IRT0940), National Natural Science Foundation of China (31272411).

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Authors and Affiliations

  1. College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Hong Wang, Gong Cheng, Changzhen Fu, Hongbao Wang, Wucai Yang, Hongcheng Wang & Linsen Zan

  2. National Beef Cattle Improvement Centre of China, Yangling, Shaanxi, People’s Republic of China

    Gong Cheng, Hongbao Wang, Wucai Yang, Hongcheng Wang & Linsen Zan

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  1. Hong Wang
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  2. Gong Cheng
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  3. Changzhen Fu
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  4. Hongbao Wang
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  6. Hongcheng Wang
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  7. Linsen Zan
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Correspondence to Linsen Zan.

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Wang, H., Cheng, G., Fu, C. et al. Sequence analysis of bovine C/EBPδ gene and its adipogenic effects on fibroblasts. Mol Biol Rep 41, 251–257 (2014). https://doi.org/10.1007/s11033-013-2858-y

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  • DOI: https://doi.org/10.1007/s11033-013-2858-y

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