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Critical role of TRPC6 channels in the development of human renal cell carcinoma

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Abstract

Renal cell carcinoma (RCC) is the most common tumor arising from the cells in the lining of tubules in the kidney. Some members of the Ca2+-permeable transient receptor potential canonical (TRPC) family of channel proteins have demonstrated a role in the proliferation of some types of cancer cells. In this study, we investigated the role of TRPC6 in the development of human RCC. RT-PCR and Western blotting were used to investigate TRPC6 expression in 1932 and ACHN cells. Immunohistochemical techniques were applied to study TRPC6 expression in 60 cases of RCC primary tissue samples and 10 cases of corresponding normal renal tissues. To inhibit TRPC6 activity or expression, RNA interference was used. The effects of TRPC6 channels on RCC cell viability and cell cycle progression were investigated by MTT and flow cytometry. TRPC6 was expressed in 1932 and ACHN cells. TRPC6 protein was detected in 73.3 % of RCC samples, and there was a significant difference compared with the normal renal samples (30 %) (p < 0.05). Moreover the level of TRPC6 expression was associated with RCC Fuhrman grade (p < 0.01). Blockade of TRPC6 channels in ACHN cells suppressed basal cell proliferation and partially inhibited HGF-induced cell proliferation. Furthermore, inhibition of TRPC6 channels expression prolonged the transition through G2/M phase in ACHN cells. In summary, expression of TRPC6 is markedly increased in RCC specimens and associated with RCC histological grade. TRPC6 plays an important role in ACHN cells proliferation.

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Acknowledgments

This study was supported by Grants (81202380) from the National Natural Science Foundation of China, Grants (20111202120016) from Ph.D. Programs Foundation of Ministry of Education of China, Grants (12JCQNJC07800) from Natural Science Foundation of Tianjin and Grants (20110138) from Tianjin Municipal Higher School Science and technology development fund program.

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Authors and Affiliations

  1. School of Laboratory Medicine, Tianjin Medical University, Tianjin, 300203, China

    Jiayin Song, Xue Li, Yanna Shen, Meilin Yin, Yunde Liu & Dan Yue

  2. Department of Urology, Tianjin Institute of Urology, Tianjin Medical University Second Hospital, Tianjin, 300201, China

    Yong Wang

  3. Department of Pathology, Tianjin Medical University Cancer Hospital, Tianjin, 300060, China

    Yuhong Guo

  4. Department of Urology, Tianjin Medical University Cancer Hospital, Tianjin, 300060, China

    Lei Diao

Authors
  1. Jiayin Song
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  2. Yong Wang
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  3. Xue Li
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  4. Yanna Shen
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  5. Meilin Yin
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  6. Yuhong Guo
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  7. Lei Diao
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  8. Yunde Liu
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  9. Dan Yue
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Corresponding authors

Correspondence to Yunde Liu or Dan Yue.

Additional information

Jiayin Song and Yong Wang both authors contributed equally to this study.

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Song, J., Wang, Y., Li, X. et al. Critical role of TRPC6 channels in the development of human renal cell carcinoma. Mol Biol Rep 40, 5115–5122 (2013). https://doi.org/10.1007/s11033-013-2613-4

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  • DOI: https://doi.org/10.1007/s11033-013-2613-4

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