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Transferability of simple sequence repeat (SSR) markers developed in guava (Psidium guajava L.) to four Myrtaceae species

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Abstract

Present study demonstrated the cross-genera transferability of 23 simple sequence repeat (SSR) primer pairs developed for guava (Psidium guajava L.) to four new targets, two species of eucalypts (Eucalyptus citriodora, Eucalyptus camaldulensis), bottlebrush (Callistemon lanceolatus) and clove (Syzygium aromaticum), belonging to the family Myrtaceae and subfamily Myrtoideae. Off the 23 SSR loci assayed, 18 (78.2 %) gave cross-amplification in E. citriodora, 14 (60.8 %) in E. camaldulensis and 17-17 (73.9 %) in C. lanceolatus and S. aromaticum. Eight primer pairs were found to be transferable to all four species. The number of alleles detected at each locus ranged from one to nine, with an average of 4.8, 2.6, 4.5 and 4.6 alleles in E. citriodora, E. camaldulensis, C. lanceolatus and S. aromaticum, respectively. The high levels of cross-genera transferability of guava SSRs may be applicable for the analysis of intra- and inter specific genetic diversity of target species, especially in E. citriodora, C. lanceolatus and S. aromaticum, for which till date no information about EST-derived as well as genomic SSR is available.

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References

  1. Varshney RK, Graner A, Sorrells ME (2005) Genic microsatellite markers in plants: features and applications. Trends Biotechnol 23:48–55

    Article  PubMed  CAS  Google Scholar 

  2. Powell W, Machray GC, Provan J (1996) Polymorphism revealed by simple sequence repeats. Trends Plant Sci 1:215–222

    Google Scholar 

  3. Kalia RK, Rai MK, Kalia S, Singh R, Dhawan AK (2011) Microsatellites markers: an overview of the recent progress in plants. Euphytica 177:309–334

    Article  CAS  Google Scholar 

  4. Gutierrez MV, Vaz Patto MC, Huguet T, Cubero JI, Moreno MT, Torres AM (2005) Cross-species amplification of Medicago truncatula microsatellites across three major pulse crops. Theor Appl Genet 110:1210–1217

    Article  PubMed  CAS  Google Scholar 

  5. Kuleung C, Baenziger PS, Dweikat I (2004) Transferability of SSR markers among wheat, rye, and triticale. Theor Appl Genet 108:1147–1150

    Article  PubMed  CAS  Google Scholar 

  6. Varshney RK, Sigmund R, Borner A, Korzun V, Stein N, Sorrells ME, Langridge P, Graner A (2005) Interspecific transferability and comparative mapping of barley EST-SSR markers in wheat rye and rice. Plant Sci 168:195–202

    Article  CAS  Google Scholar 

  7. Ellis JR, Burke JM (2007) EST-SSRs as a resource for population genetic analyses. Heredity 99:125–132

    Article  PubMed  CAS  Google Scholar 

  8. Souza LM, Mantello CC, Santos MO, de Souza Goncalves P, Souza AP (2009) Microsatellites from rubber tree (Hevea brasiliensis) for genetic diversity analysis and cross-amplification in six Hevea wild species. Conserv Genet Resour 1:75–79

    Article  Google Scholar 

  9. Ekue MRM, Gailing O, Finkeldey R (2009) Transferability of simple sequence repeat (SSR) markers developed in Litchi chinensis to Blighia sapida (Sapindaceae). Plant Mol Biol Rep 27:570–574

    Article  CAS  Google Scholar 

  10. Mnejja M, Garcia-Mas J, Audergon JM, Arus P (2010) Prunus microsatellite marker transferability across rosaceous crops. Tree Genet Genome 6:689–700

    Article  Google Scholar 

  11. Sudheer PDVN, Mastan SG, Rahman H, Ravi Prakash C, Singh S, Reddy MP (2011) Cross species amplification ability of novel microsatellites isolated from Jatropha curcas and genetic relationship with sister taxa. Mol Biol Rep 38:1383–1388

    Article  PubMed  CAS  Google Scholar 

  12. Yu F, Wang BH, Feng SP, Wang JY, Li WG, Wu YT (2011) Development, characterization, and cross-species/genera transferability of SSR markers for rubber tree (Hevea brasiliensis). Plant Cell Rep 30:335–344

    Article  PubMed  CAS  Google Scholar 

  13. Gupta S, Kumari K, Sahu PP, Vidapu S, Prasad M (2012) Sequence-based novel genomic microsatellite markers for robust genotyping purposes in foxtail millet [Setaria italica (L.) P. Beauv]. Plant Cell Rep 31:323–337

    Article  PubMed  CAS  Google Scholar 

  14. Harris-Shultz KR, Milla-Lewis SR, Brady JA (2012) Transferability of SSR and RGA markers developed in Cynodon spp. to Zoysia spp. Plant Mol Biol Rep 30:1264–1269

    Article  CAS  Google Scholar 

  15. Gasic K, Han Y, Kertbundit S, Shulaev V, Iezzoni AF, Stover EW, Richard L, Michael B, Wisniewski E, Korban SS (2009) Characteristics and transferability of new apple EST-derived SSRs to other Rosaceae species. Mol Breed 23:397–411

    Article  CAS  Google Scholar 

  16. Ince AG, Karaca M, Onus AN (2010) Polymorphic microsatellite markers transferable across Capsicum species. Plant Mol Biol Rep 28:285–291

    Article  CAS  Google Scholar 

  17. Grattapaglia D, Vaillancourt RE, Shepherd M, Thumma BR, Foley W, Külheim C, Potts BM, Myburg AA (2012) Progress in Myrtaceae genetics and genomics: Eucalyptus as the pivotal genus. Tree Genet Genome 8:463–508

    Article  Google Scholar 

  18. Dirlewanger E, Graziano E, Joobeur T, Garriga-Caldere F, Cosson P, Howad W, Arus P (2004) Comparative mapping and marker-assisted selection in Rosaceae fruit crops. Proc Natl Acad Sci USA 101:9891–9896

    Article  PubMed  CAS  Google Scholar 

  19. Rossetto M, Harriss FCL, McLauchlan A, Henry RJ, Baverstock PR, Lee LS (2000) Interspecific amplification of tea tree (Melaleuca alternifolia—Myrtaceae) microsatellite loci—potential implications for conservation studies. Aust J Bot 48:367–373

    Article  Google Scholar 

  20. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15

    Google Scholar 

  21. Gupta AK, Harish, Rai MK, Phulwaria M, Shekhawat NS (2011) Isolation of genomic DNA suitable for community analysis from mature trees adapted to arid environment. Gene 487:156–159

    Article  PubMed  CAS  Google Scholar 

  22. Risterucci AM, Duval MF, Rohde W, Billotte N (2005) Isolation and characterization of microsatellite loci from Psidium guajava L. Mol Ecol Notes 5:745–748

    Article  CAS  Google Scholar 

  23. Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32(3):314–331

    PubMed  CAS  Google Scholar 

  24. Saha MC, Cooper JD, Rouf Mian MA, Chekhovskiy K, May GD (2006) Tall fescue genomic SSR markers: development and transferability across multiple grass species. Theor Appl Genet 113:1449–1458

    Article  PubMed  CAS  Google Scholar 

  25. Viruel MA, Hormaza JI (2004) Development, characterization and variability analysis of microsatellites in lychee (Litchi chinensis Sonn., Sapindaceae). Theor Appl Genet 108:896–902

    Article  PubMed  CAS  Google Scholar 

  26. Kanupriya, Madhavi Latha P, Aswath C, Reddy L, Padmakar B, Vasugi C, Dinesh MR (2011) Cultivar identification and genetic fingerprinting of guava (Psidium guajava) using microsatellite markers. Int J Fruit Sci 11:184–196

    Article  Google Scholar 

  27. Zhang Q, Li J, Zhao Y, Korban SS, Han Y (2012) Evaluation of genetic diversity in Chinese wild apple species along with apple cultivars using SSR markers. Plant Mol Biol Rep 30:539–546

    Article  CAS  Google Scholar 

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Acknowledgments

The authors (MKR and MP) acknowledge to University Grants Commission (UGC), New Delhi for the award of Dr. D.S. Kothari Post Doctoral Fellowship and UGC Post Doctoral Fellowship. We are thankful to Mr. Satish Kumar Verma, Mycopathology and Microbial Technology Laboratory, Department of Botany, BHU, Varanasi for providing plant materials.

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Authors and Affiliations

  1. Department of Botany, Biotechnology Centre, Jai Narain Vyas University, Jodhpur, 342033, Rajasthan, India

    Manoj K. Rai, Mahendra Phulwaria & N. S. Shekhawat

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  1. Manoj K. Rai
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  2. Mahendra Phulwaria
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  3. N. S. Shekhawat
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Correspondence to Manoj K. Rai.

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Rai, M.K., Phulwaria, M. & Shekhawat, N.S. Transferability of simple sequence repeat (SSR) markers developed in guava (Psidium guajava L.) to four Myrtaceae species. Mol Biol Rep 40, 5067–5071 (2013). https://doi.org/10.1007/s11033-013-2608-1

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  • DOI: https://doi.org/10.1007/s11033-013-2608-1

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