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The polymorphisms of LYRM1 gene and their association with body measurement and ultrasound traits of Qinchuan cattle

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Abstract

Body measurement and meat quality traits which play important roles in the assessment of productivity and economy in cattle were influenced by genes and environmental factors. Latest studies showed that LYR motif containing 1 (LYRM1) may be involved in influencing fatness deposition in animals. The objective of this study was to detect bovine LYRM1 gene polymorphism and analyze its association with body measurement and meat quality traits of cattle. Blood samples were taken from a total of 404 Qinchuan cattle aged from 18–24 months. Created restriction site-polymerase chain reaction-restriction fragment length polymorphism (CRS-PCR–RFLP) and DNA sequencing were used to find out LYRM1 single polymorphism nucleotide (SNPs). Sequence analysis of LYRM1 gene revealed two SNPs (g.165 C > A, g.193 A > G) in 3′ untranslated region (3′UTR) of exon 3. And g.165 C > A showed two genotypes namely AC and CC while g.193 A > G showed three genotypes: AA, AG and GG. Analysis results showed that there were significant associations between polymorphism of these two and body measurement and meat quality traits in Qinchuan cattle population. Based on the results obtained from this study, it is inferred that LYRM1 gene may have potential effects on body measurement and meat quality traits in Qinchuan cattle population and could be used for marker-assisted selection.

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Acknowledgments

We thank all the research assistants and laboratory technicians who contributed to this study. We extend special thanks to Professor Zan and Dr. Cheng for their assistance. This work was supported by Program for Changjiang Scholars and Innovative Research Team (IRT0940), beneficial beef cattle and cellular engineer breeding technology research (2011AA100307-02), selecting and breeding new high quality beef cattle and its key technologies (2011BAD28B04-03), GMO new varieties major project (2011ZX08007-002), Beef cattle and Yak Industrial Technology System Project (CARS-38).

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Authors and Affiliations

  1. College of Animal Science and Technology, Northwest A & F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Yaokun Li, Jianbin Gao, Ning Yang, Changzhen Fu, Gong Cheng, Hongbao Wang & Linsen Zan

  2. National Beef Cattle Improvement Center of Northwest A & F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Gong Cheng, Hongbao Wang & Linsen Zan

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  1. Yaokun Li
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  2. Jianbin Gao
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  3. Ning Yang
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  4. Changzhen Fu
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  5. Gong Cheng
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  6. Hongbao Wang
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  7. Linsen Zan
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Correspondence to Linsen Zan.

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Li, Y., Gao, J., Yang, N. et al. The polymorphisms of LYRM1 gene and their association with body measurement and ultrasound traits of Qinchuan cattle. Mol Biol Rep 40, 1511–1517 (2013). https://doi.org/10.1007/s11033-012-2195-6

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  • DOI: https://doi.org/10.1007/s11033-012-2195-6

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