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Cloning, identification and expression analysis of ACC oxidase gene involved in ethylene production pathway

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Abstract

1-aminocyclopropane-1-carboxylic acid oxidase (ACO) enzyme is a member of the Fe II-dependent family of oxidases/oxygenases which require Fe2+ as a cofactor, ascorbate as a cosubstrate and CO2 as an activator. This enzyme catalyses the terminal step in the plant signaling of ethylene biosynthetic pathway. A 948 bp fragment of the ACO1 gene cDNA sequence was cloned from tomato (Lycopersicon esculentum) fruit tissues by using reverse transcriptase-polymerase chain reaction (RT-PCR) with two PCR primers designed according to the sequence of a tomato cDNA clone (X58273). The BLAST search showed a high level of similarity (77–98 %) between ACO1 and ACO genes of other plants. The calculated molecular mass and predicted isoelectric point of LeACO1 were 35.8 kDa and 5.13, respectively. The three-dimensional structure studies illustrated that the LeACO1 protein folds into a compact jelly-roll motif comprised of 8 α-helices, 12 β-strands and several long loops. The cosubstrate was located in a cofactor-binding pocket referred to as a 2-His-1-carboxylate facial triad. Semi-quantitative RT-PCR analysis of gene expression revealed that the LeACO1 was expressed in fruit tissues at different ripening stages.

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Abbreviations

ACC:

1-Aminocyclopropane-1-carboxylic acid

ACO:

ACC oxidase

ACS:

ACC synthase

SAM:

S-adenosylmethionine

ORF:

Open reading frame

RT-PCR:

Reverse transcription polymerase chain reaction

Bp:

Base pair

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, Imam Khomeini International University, P.O. Box 34149-288, Qazvin, Islamic Republic of Iran

    Zohreh Jafari, Raheem Haddad, Ramin Hosseini & Ghasemali Garoosi

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  1. Zohreh Jafari
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  2. Raheem Haddad
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  3. Ramin Hosseini
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  4. Ghasemali Garoosi
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Correspondence to Raheem Haddad.

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Jafari, Z., Haddad, R., Hosseini, R. et al. Cloning, identification and expression analysis of ACC oxidase gene involved in ethylene production pathway. Mol Biol Rep 40, 1341–1350 (2013). https://doi.org/10.1007/s11033-012-2178-7

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  • DOI: https://doi.org/10.1007/s11033-012-2178-7

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