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Characterization of a vacuolar zinc transporter OZT1 in rice (Oryza sativa L.)

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Abstract

The CDF family is a ubiquitous family that has been identified in prokaryotes, eukaryotes, and archaea. Members of this family are important heavy metal transporters that transport metal ions out of the cytoplasm. In this research, a full length cDNA named Oryza sativa Zn Transporter 1 (OZT1) that closely related to rat ZnT-2 (Zn Transporter 2) gene was isolated from rice. The OZT1 encoding a CDF family protein shares 28.2 % ~ 84.3 % of identities and 49.3 % ~ 90.9 % of similarities with other zinc transporters such as RnZnT-2, HsZnT-8, RnZnT-8 and AtMTP1. OZT1 was constitutively expressed in various rice tissues. The OZT1 expression was significantly induced both in the seedlings of japonica rice Nipponbare and indica rice IR26 in response to Zn2+ and Cd2+ treatments. Besides, OZT1 expression was also increased when exposed to other excess metals, such as Cu2+, Fe2+ and Mg2+. Subcellular localization analysis indicated that OZT1 localized to vacuole. Heterologous expression of OZT1 in yeast increased tolerance to Zn2+ and Cd2+ stress but not the Mg2+ stress. Together, OZT1 is a CDF family vacuolar zinc transporter conferring tolerance to Zn2+ and Cd2+ stress, which is important to transporting and homeostasis of Zn, Cd or other heavy metals in plants.

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Abbreviations

ABC:

ATP binding cassette

CDF:

Cation diffusion facilitator

OZT:

Oryza sativa zinc transporter protein

Nramp:

Natural resistance-associated macrophage protein

ZIP:

ZRT, IRT-like protein

TM:

Transmembrane domain

LZM:

Leu zipper motif

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Acknowledgments

We were indebted to David Eide providing the yeast strains DY1455 and yeast expression vector pFL61. This work was supported by National Natural Science Foundation of China (Nos. 30971556, 31071397), State Key Laboratory of Rice Biology (No. 110101) and Fundamental Research Funds for the Central Universities (No. KYZ201137).

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Hong-Xia Lan, Zhou-Fei Wang, Qi-Hong Wang, Mei-Mei Wang, Yong-Mei Bao, Ji Huang & Hong-Sheng Zhang

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  1. Hong-Xia Lan
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  2. Zhou-Fei Wang
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Correspondence to Ji Huang or Hong-Sheng Zhang.

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Hong-Xia Lan and Zhou-Fei Wang contributed equally to this work.

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Lan, HX., Wang, ZF., Wang, QH. et al. Characterization of a vacuolar zinc transporter OZT1 in rice (Oryza sativa L.). Mol Biol Rep 40, 1201–1210 (2013). https://doi.org/10.1007/s11033-012-2162-2

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