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Comparative transcriptome analysis of tobacco (Nicotiana tabacum) leaves to identify aroma compound-related genes expressed in different cultivated regions

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Abstract

To identify genes that are differentially expressed in tobacco in response to environmental changes and to decipher the mechanisms by which aromatic carotenoids are formed in tobacco, an Agilent Tobacco Gene Expression microarray was adapted for transcriptome comparison of tobacco leaves derived from three cultivated regions of China, Kaiyang (KY), Weining (WN) and Tianzhu (TZ). A total of 1,005 genes were differentially expressed between leaves derived from KY and TZ, 733 between KY and WN, and 517 between TZ and WN. Genes that were upregulated in leaves from WN and TZ tended to be involved in secondary metabolism pathways, and included several carotenoid pathway genes, e.g., NtPYS, NtPDS, and NtLCYE, whereas those that were down-regulated tended to be involved in the response to temperature and light. The expression of 10 differentially expressed genes (DEGs) was evaluated by real-time quantitative polymerase chain reaction (qRT-PCR) and found to be consistent with the microarray data. Gene Ontology and MapMan analyses indicate that the genes that were differentially expressed among the three cultivated regions were associated with the light reaction of photosystem II, response to stimuli, and secondary metabolism. High-performance liquid chromatography (HPLC) analysis showed that leaves derived from KY had the lowest levels of lutein, β-carotene, and neoxanthin, whereas the total carotenoid content in leaves from TZ was greatest, a finding that could well be explained by the expression patterns of DEGs in the carotenoid pathway. These results may help elucidate the molecular mechanisms underlying environmental adaptation and accumulation of aroma compounds in tobacco.

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Abbreviations

a.s.l.:

Above sea level

DEG:

Differentially expressed gene

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Acknowledgments

This work was supported by grants from the Key Special Program of China National Tobacco Corporation (TS-02-20110014), the Program of Guizhou Provincial Tobacco Company (2007-04 and 2010-02), the Foundation of Science and Technology of Guizhou Province of China (J[2010]2088), the Fundamental Research Funds for the Central Universities (XDJK2009B009 and XDJK2012A009), the Specialized Research Fund for the Doctoral Program of Higher Education of China (20100182120016), the “111” Project (B12006) and the Natural Science Foundation of Chongqing of China (cstc2011jjA80026).

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Authors and Affiliations

  1. Guizhou Tobacco Research Institute, North Yuntan Road, Jinyang New District, Guiyang, 550081, People’s Republic of China

    Bo Lei, Jie Zhang, Zhu Ren, Yi Chen, Hui-Na Zhao, Wen-Jie Pan, Wei Chen, Hong-Xun Li & Fu-Zhang Ding

  2. Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing, People’s Republic of China

    Xue-Hua Zhao, Kai Zhang, Wen-Ya Deng & Kun Lu

  3. College of Agronomy and Biotechnology, Southwest University, Tiansheng Road 2, Beibei, Chongqing, 400715, People’s Republic of China

    Xue-Hua Zhao, Kai Zhang, Wen-Ya Deng & Kun Lu

  4. School of Computer and Information Science, Southwest University, Tiansheng Road 2, Beibei, Chongqing, 400715, People’s Republic of China

    Wei Ren

  5. Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN, 47907, USA

    Kun Lu

Authors
  1. Bo Lei
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  2. Xue-Hua Zhao
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  10. Wei Chen
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  12. Wen-Ya Deng
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  13. Fu-Zhang Ding
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  14. Kun Lu
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Corresponding authors

Correspondence to Fu-Zhang Ding or Kun Lu.

Additional information

Bo Lei, Xue-Hua Zhao, Kai Zhang and Fu-Zhang Ding equally contributed to this study.

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Lei, B., Zhao, XH., Zhang, K. et al. Comparative transcriptome analysis of tobacco (Nicotiana tabacum) leaves to identify aroma compound-related genes expressed in different cultivated regions. Mol Biol Rep 40, 345–357 (2013). https://doi.org/10.1007/s11033-012-2067-0

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  • DOI: https://doi.org/10.1007/s11033-012-2067-0

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