Skip to main content
SpringerLink
Log in

Genetic diversity study of some medicinal plant accessions belong to Apiaceae family based on seed storage proteins patterns

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Cumin (Cuminum cyminum L.), Fennel (Foeniculum vulgare L.) and Longleaf (Falcaria vulgaris Bernh) that all belong to Apiaceae family as medicinal plants are very important in many countries. Study of genetic diversity for medicinal plant is important for researches in future. One of the methods to evaluate plant genetic diversity and classification of them is the electrophoresis of seed storage proteins. This research was conducted in order to evaluate seed protein variability in different Iranian Cumin, Fennel and Longleaf accessions and grouping them based on these proteins as a biochemical marker. For this purpose, the samples were first powdered in liquid nitrogen and seed protein was extracted with extraction buffer. Then total soluble proteins were resolved on 12.5 % sodium dodecyl sulphate polyacrylamide gel electrophoresis gels. The electrophoretic protein pattern showed 38 bands that were low polymorphism among the accessions. The result of cluster analysis showed that the accessions were classified in three groups (all 29 Cumin accessions in the first group, three Fennel ecotypes in second group and three Longleaf accessions in the last one).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Heywood VH (1971) The biology and chemistry of the Umbelliferae. Academic press, London

    Google Scholar 

  2. Omidbaigi R (2007) Production and processing of medicinal plants. Tarbiat Modares University Press, Tehran

    Google Scholar 

  3. Kafi M (2003) Cumin production and processing. Ferdowsi University of Mashhad Press, Mashhad

    Google Scholar 

  4. Akgul A (1986) Studies on the essential oils from Turkish fennel seeds (Foeniculum vulgare M. var. dulce). Prog Essent Oil Res 4:319–323

    Google Scholar 

  5. Khazaei M, Salehi H (2006) Protective effect of Falcaria vulgaris extract on ethanol induced gastric ulcer in rat. Iran J Pharm Ther 5:43–46

    Google Scholar 

  6. Rahimmalek M, Bahreininejad B, Khorrami M, Sayed Tabatabaaei BE (2009) Genetic variability and geographic differentiation in Thymus daenensis, an endangered medicinal plant, as revealed by inter simple sequence repeat (ISSR). Biochem Genet 47:831–842

    Article  PubMed  CAS  Google Scholar 

  7. Kakaei M, Kahrizi D (2011) Study of seed proteins pattern of Brassica napus varieties via sodium dodecyl sulfate polyacrylamide gel electrophoresis. Int Res J Biotechnol 2:26–28

    Google Scholar 

  8. Ravi M, Geethanjali SF, Sameeyafarheen FM, Maheswaran M (2003) Molecular marker based genetic diversity analysis in rice (Oryza sativa L.) using RAPD and SSR markers. Euphytica 133:243–252

    Article  CAS  Google Scholar 

  9. Inam U, Ahmed Khan I, Ahmed H, Ghafoor S, Gul S, Muhammad I, Ilyas M (2010) Seed storage protein profile of rice varieties commonly grown in Pakistan. Asian J Agric Sci 2:120–123

    Google Scholar 

  10. Rostami-Ahmadvandi H, Kahrizi D, Zebarjadi AR, Mostafaie A, Sohrabi-Babahadi F, Kiani S (2011) Study of Peganum harmala genetic diversity based on sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. J Agric Sci Technol A 1:1300–1302

    Google Scholar 

  11. Shuaib M, Zeb A, Ali Z, Ali W, Ahmad T, Khan I (2007) Characterization of wheat varieties by seed storage protein electrophoresis. Afr J Biotechnol 6:497–500

    CAS  Google Scholar 

  12. Valizadeh M (2001) Seed storage protein profile of grain legumes grown in Iran, using SDS-PAGE. J Agric Sci Technol 3:287–292

    Google Scholar 

  13. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  PubMed  CAS  Google Scholar 

  14. Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

  15. Xi J, Wang X, Li S, Zhou X, Yue L, Fan J, Hao D (2006) Polyethylene glycol fractionation improved detection of low abundant proteins by two-dimensional electrophoresis analysis of plant proteome. Phytochemistry 67:2341–2348

    Article  PubMed  CAS  Google Scholar 

  16. Iqbal SH, Ghafoor A, Ayub N (2005) Relationship between SDS-PAGE markers and Ascochyta blight in chickpea. Pak J Bot 37:87–96

    Google Scholar 

  17. Javid A, Ghafoor A, Anwar R (2004) Seed storage protein electrophoresis in groundnut for evaluating genetic diversity. Pak J Bot 36:25–29

    Google Scholar 

  18. Shoorideh H (2004) Evaluation of some indicators of drought resistance in Cumin (Cuminum cyminum L.) and introduce its genotype using storage protein electrophoresis. Dissertation, Shiraz University

  19. Fracaro F, Echeverrigaray S (2006) Genetic variability in Hesperozygis Ringens Benth. (Lamiaceae) an endangered aromatic and medicinal plant of southern Brazil. Biochem Genet 44:479–490

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to gratefully acknowledge all who help us for gathering accessions from different regions.

Author information

Authors and Affiliations

  1. Department of Natural Resources, Razi University, Kermanshah, Iran

    Sayed Mohammad Masoumi

  2. Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran

    Danial Kahrizi, Hossein Rostami-Ahmadvandi & Jahad Soorni

  3. Department of Biotechnology for Drought Tolerance Research, Razi University, Kermanshah, Iran

    Danial Kahrizi

  4. Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Sara Kiani, Ali Mostafaie & Kheirollah Yari

Authors
  1. Sayed Mohammad Masoumi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Danial Kahrizi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hossein Rostami-Ahmadvandi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jahad Soorni
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sara Kiani
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ali Mostafaie
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Kheirollah Yari
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Danial Kahrizi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Masoumi, S.M., Kahrizi, D., Rostami-Ahmadvandi, H. et al. Genetic diversity study of some medicinal plant accessions belong to Apiaceae family based on seed storage proteins patterns. Mol Biol Rep 39, 10361–10365 (2012). https://doi.org/10.1007/s11033-012-1914-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-012-1914-3

Keywords

Search

Navigation