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The investigation of genetic polymorphisms in the carbonic anhydrase VI gene exon 2 and salivary parameters in type 2 diabetic patients and healthy adults

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Abstract

The aim of this study was to investigate carbonic anhydrase (CA) VI Exon 2 single nucleotide polymorphism (SNP) and its possible association with salivary parameters in type 2 diabetic patients compared to healthy adults. Caries status was measured by using the DMFT (number of decayed, missing, and filled teeth) index. Unstimulated whole saliva and blood samples were taken. SNPs of CA gene exon 2 were determined by PCR and DNA sequencing. Salivary CA activity and buffering capacity were determined by the method of Verpoorte and Ericson, respectively. Furthermore, salivary pH was measured with pH paper and salivary flow rate was calculated. Salivary buffering capacity and pH were significantly lower in diabetic patients than those of healthy subjects (P < 0.05). Salivary flow rate, CA activity and DMFT levels did not differ between groups (P > 0.05). Four SNPs were detected; their pubmed database number are rs2274327 (C/T), rs2274328 (A/C), rs2274329 (G/C) and rs2274330. While first three of those were responsible for amino acid changes, the last one was not. The frequencies of SNPs were not significant between groups (P > 0.05). Positive significant correlation was found between CA activity and the frequency of SNPs. There was no correlation between the SNPs frequencies and pH or buffering capacity. SNPs found in this study may be related to salivary CA activity in diabetics.

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Acknowledgment

This study was financially supported by Grant Project No: SAG.YY.009/140604 from the Commision of Scientific Investigations Projects of Marmara University.

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Authors and Affiliations

  1. Basic Medical Sciences, Department of Biochemistry, Faculty of Dentistry, Marmara University, Nisantasi, 34365, Istanbul, Turkey

    Leyla Koç Öztürk, Korkut Ulucan & Ayşen Yarat

  2. Department of Clinical Sciences, Faculty of Dentistry, Marmara University, Nisantasi, 34365, Istanbul, Turkey

    Serap Akyüz

  3. Department of Clinical Sciences, Faculty of Dentistry, Ondokuz Mayis University, Samsun, Turkey

    Halit Furuncuoğlu

  4. Turkish Diabetes Association, Harbiye, Istanbul, Turkey

    Hikmet Bayer

Authors
  1. Leyla Koç Öztürk
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  2. Korkut Ulucan
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  3. Serap Akyüz
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  4. Halit Furuncuoğlu
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  5. Hikmet Bayer
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  6. Ayşen Yarat
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Correspondence to Leyla Koç Öztürk.

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Koç Öztürk, L., Ulucan, K., Akyüz, S. et al. The investigation of genetic polymorphisms in the carbonic anhydrase VI gene exon 2 and salivary parameters in type 2 diabetic patients and healthy adults. Mol Biol Rep 39, 5677–5682 (2012). https://doi.org/10.1007/s11033-011-1374-1

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  • DOI: https://doi.org/10.1007/s11033-011-1374-1

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