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The effects of NOS3 Glu298Asp variant on colorectal cancer risk and progression in Turkish population

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Abstract

Endothelial nitric oxide synthase (eNOS), coded by the gene NOS3, may play an important role in uncontrollable cellular growth in several cancer types. Our study was performed to test the association between Glu298Asp polymorphisms in the NOS3 gene and colorectal cancer risk and progression. In this study, NOS3 Glu298Asp polymorphism was genotyped in 84 patients with colorectal cancer and 99 healthy subjects using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) analysis. There were significant differences in the distribution of NOS3 genotypes and frequencies of the alleles between colorectal cancer patients and controls (P = 0.016, P = 0.006, respectively). The increased frequency of NOS3 Glu298Asp homozygotes genotypes in patients who had advanced tumour stage was statistically significant (P = 0.042). Our findings have suggested that NOS3 Glu298Asp polymorphism might be associated with the risk and progression of colorectal cancer in Turkish population.

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Acknowledgments

This study was supported by a grant from Istanbul University, Research Foundation (Project 488/05052006), Turkey. We are also grateful to the cancer epidemiologist Dr. Hakan Camlica for support and correction of statistical analysis.

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Authors and Affiliations

  1. Surgery Clinic, Istanbul Education and Research Hospital, Istanbul, Turkey

    Soykan Arıkan, Erkan Guler & Serdar Çulcu

  2. Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, P.O. Box 7, 34390 Çapa, Istanbul, Turkey

    Canan Cacina, Gulay Tuna & İlhan Yaylım-Eraltan

Authors
  1. Soykan Arıkan
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  2. Canan Cacina
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  3. Erkan Guler
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  4. Serdar Çulcu
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  6. İlhan Yaylım-Eraltan
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Correspondence to İlhan Yaylım-Eraltan.

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Arıkan, S., Cacina, C., Guler, E. et al. The effects of NOS3 Glu298Asp variant on colorectal cancer risk and progression in Turkish population. Mol Biol Rep 39, 3245–3249 (2012). https://doi.org/10.1007/s11033-011-1092-8

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  • DOI: https://doi.org/10.1007/s11033-011-1092-8

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