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An MspI polymorphism in the inhibin alpha gene and its associations with superovulation traits in Chinese Holstein cows

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Abstract

To identify a predictor to forecast superovulation response on the basis of associations between superovulation performance and gene polymorphism, the PCR–RFLP method was applied to detect an A>G transition determining an MspI polymorphism at position 192 in the exon I of the bovine inhibin alpha (INHA) gene and evaluate its associations with superovulatory response in 118 Chinese Holstein cows treated for superovulation. Association analysis showed that cows with the GG genotype resulted in a significant increase in the number of ova (TNO) than AG and AA genotypes in the first (P = 0.023), second (P = 0.004) and third (P = 0.002) superovulation treatments and produced more transferable embryos (NTE) than that of AG and AA genotypes in the third (P = 0.045) superovulation treatment. Moreover, individuals with GG genotype produced more transferable embryos than AA (P < 0.05) genotype in the second superovulation treatment and all cows without superovulation response were mutations with genotypes of AA and AG. These results indicate that INHA gene can be used as a predictor for superovulation in Chinese Holstein cows, and imply that cows with AA genotype should be excluded for superovulation practices.

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Acknowledgments

This work was supported by both of the National High Technology Research and Development Program of China (863 program) (No.2007AA10Z152) and National Key Technology R&D Program (No. 2006BAD04A02-11).

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Authors and Affiliations

  1. Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Ke-Qiong Tang, Wu-Cai Yang, Jun-Na Yu, Li Han, Xiang Li & Li-Guo Yang

  2. Beijing Amber Embryo Technology Co. Ltd, Beijing, 100107, China

    Shu-Jing Li

  3. College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Li-Guo Yang

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  1. Ke-Qiong Tang
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  2. Shu-Jing Li
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  3. Wu-Cai Yang
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  4. Jun-Na Yu
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  5. Li Han
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  6. Xiang Li
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  7. Li-Guo Yang
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Correspondence to Li-Guo Yang.

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Tang, KQ., Li, SJ., Yang, WC. et al. An MspI polymorphism in the inhibin alpha gene and its associations with superovulation traits in Chinese Holstein cows. Mol Biol Rep 38, 17–21 (2011). https://doi.org/10.1007/s11033-010-0072-8

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  • DOI: https://doi.org/10.1007/s11033-010-0072-8

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