Skip to main content

Advertisement

SpringerLink
Log in

Characteristics of X- and Y-chromosome specific regions of the amelogenin gene and a PCR-based method for sex identification in red deer (Cervus elaphus)

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

The present study attempts to analyse sequences of the X- and Y-chromosome specific regions of the amelogenin (AMEL) gene in red deer. To this end, primers specific for each form of the gene (AMELX and AMELY) were designed based on bovine genomic sequences and the homologous regions of the genes were sequenced. The obtained sequence of AMELX gene showed high similarity with the corresponding region in cattle (91%) and humans (77%), but this similarity was slightly lower among AMELY genes and showed 87 and 73% of identical nucleotides, respectively. In addition, three single nucleotide polymorphisms (SNPs) were found in the AMELX gene of the female red deer investigated. Comparative analysis of the homologous fragments of the red deer AMELX and AMELY genes confirmed the deletion of an AMELY gene fragment in relation to AMELX. Homology of both sequences was 82% of identical nucleotides in the coding region and 74% in 3′ non-coding sequence. The sequences studied showed considerable similarity to homologous fragments of the human and bovine gene, but the structural differences observed lead us to design PCR-based method for sex identification in red deer, based on the presented sequences.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Shimokawa H, Tamura H, Ibaraki K, Sasaki S (1989) Human amelogenin gene. In: Fearnhead RW (ed) Tooth enamel V. Florence Publsihers, Yokohama, pp 301–305

    Google Scholar 

  2. Girondot M, Sire JY (1998) Evolution of the amelogenin gene in toothed and toothless vertebrates. Eur J Oral Sci 106:501–508

    CAS  PubMed  Google Scholar 

  3. Li W, Mathews C, Gao C, DenBesten PK (1998) Identification of two additional exons at the 3′ end of the amelogenin gene. Arch Oral Biol 43:497–504

    Article  CAS  PubMed  Google Scholar 

  4. Baba O, Takahashi N, Terashima T, Li W, DenBesten PK, Takano Y (2002) Expression of alternatively spliced RNA transcripts of amelogenin gene exons 8 and 9 and its end products in the rat incisor. J Histochem Cytochem 50:1229–1236

    CAS  PubMed  Google Scholar 

  5. Nakahori Y, Takenaka O, Nakagome Y (1991) A human X–Y homologous region encodes amelogenin. Genomics 9:264–269

    Article  CAS  PubMed  Google Scholar 

  6. Sullivan KM, Mannucci A, Kimpton CP, Gill P (1993) A rapid and quantitative DNA sex test: fluorescence-based PCR analysis of X–Y homologous gene amelogenin. BioTechniques 15:636–641

    CAS  PubMed  Google Scholar 

  7. Gibson C, Golub E, Herold R, Risser M, Ding W, Shimokawa H, Young M, Termine J, Rosenbloom J (1991) Structure and expression of the bovine amelogenin gene. Biochemistry 30:1075–1079

    Article  CAS  PubMed  Google Scholar 

  8. Gibson CW, Golub EE, Abrams WR, Shen G, Ding W, Rosenbloom J (1992) Bovine amelogenin message heterogeneity: alternative splicing and Y-chromosome gene transcription. Biochemistry 31:8384–8388

    Article  CAS  PubMed  Google Scholar 

  9. Pajares G, Álvarez I, Fernández I, Pérez-Pardal L, Goyache F, Royo LJ (2007) A sexing protocol for wild ruminants based on PCR amplification of amelogenin genes AMEL and AMELY. Arch Tierz Dummerstorf 50:442–446

    CAS  Google Scholar 

  10. Ennis S, Galagher TF (1994) A PCR-based sex determination assay in cattle based on the bovine amelogenin locus. Anim Genet 25:425–427

    Article  CAS  PubMed  Google Scholar 

  11. Yamauchi K, Hamasaki S, Miyazaki K, Kikusui T, Takeuchi Y, Mori Y (2000) Sex determination based on fecal DNA analysis of the amelogenin gene in Sika Deer (Cervus nippon). J Vet Med Sci 62:669–671

    Article  CAS  PubMed  Google Scholar 

  12. Pfeiffer I, Brenig B (2005) X- and Y-chromosome specific variants of the amelogenin gene allow sex determination in sheep (Ovis aries) and European red deer (Cervus elaphus). BMC Genet 6:16–17

    Article  CAS  PubMed  Google Scholar 

  13. Sasaki S, Shimokawa H (1995) The amelogenin gene. Int J Dev Biol 39:127–133

    CAS  PubMed  Google Scholar 

  14. Richard B, Delgado S, Gorry P, Sire J (2007) A study of polymorphism in human AMELX. Arch oral Boil 52:1026–1031

    Article  CAS  Google Scholar 

  15. Sipos R, Székely AJ, Palatinszky M, Révész S, Márialigeti K, Nikolausz M (2007) Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targeting bacterial community analysis. FEMS Microbiol Ecol 60:341–350

    Article  CAS  PubMed  Google Scholar 

  16. Bru D, Martin-Laurent F, Philippot L (2008) Quantification of the detrimental effect of a single primer-template mismatch by real-time PCR using the 16S rRNA gene as an example. Appl Environ Microbiol 74:1660–1663

    Article  CAS  PubMed  Google Scholar 

  17. Findlay I, Quirke P (1996) Fluorescent polymerase chain reaction: Part I. A new method allowing genetic diagnosis and DNA fingerprinting of single cells. Hum Reprod Update 2:137–152

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Animal Immuno- and Cytogenetics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice n., Krakow, Poland

    Artur Gurgul, Anna Radko & Ewa Słota

Authors
  1. Artur Gurgul
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anna Radko
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ewa Słota
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Artur Gurgul.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gurgul, A., Radko, A. & Słota, E. Characteristics of X- and Y-chromosome specific regions of the amelogenin gene and a PCR-based method for sex identification in red deer (Cervus elaphus). Mol Biol Rep 37, 2915–2918 (2010). https://doi.org/10.1007/s11033-009-9852-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-009-9852-4

Keywords

Search

Navigation