Abstract
Tumor necrosis factor, alpha-induced protein 1 (TNFAIP1) is an immediate-early response gene of endothelium induced by TNF alpha. However, little is really known concerning the TNFAIP1 expression regulation. To better understand how TNFAIP1 expression is regulated, we functionally characterized the promoter region of human TNFAIP1 gene. Deletion mutation analysis, gel electrophoretic mobility shift, and site-directed mutagenesis assays allowed the identification of one functional Sp1-binding site within the human TNFAIP1 core promoter region. Moreover, chromatin immunoprecipitation analysis indicated that Sp1 was associated in vivo with the TNFAIP1 promoter. Further, Sp1 overexpression enhanced TNFAIP1 promoter activity. These findings suggest that Sp1 is implicated in the control of basal TNFAIP1 gene expression. Accordingly, Sp1 is supposed to be involved in the elevation of TNFAIP1 in response to TNF alpha induction, and thus participate in inflammation-associated angiogenesis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Wolf FW, Marks RM, Sarma V et al (1992) Characterization of a novel tumor necrosis factor-alpha-induced endothelial primary response gene. J Biol Chem 267:1317–1326
Zhou J, Hu X, Xiong X et al (2005) Cloning of two rat PDIP1 related genes and their interactions with proliferating cell nuclear antigen. J Exp Zoolog A Comp Exp Biol 303:227–240. doi:10.1002/jez.a.150
Link CD, Taft A, Kapulkin V et al (2003) Gene expression analysis in a transgenic Caenorhabditis elegans Alzheimer’s disease model. Neurobiol Aging 24:397–413. doi:10.1016/S0197-4580(02)00224-5
Zhou J, Ren K, Liu X et al (2005) A novel PDIP1-related protein, KCTD10, that interacts with proliferating cell nuclear antigen and DNA polymerase delta. Biochim Biophys Acta 1729:200–203
Yang LP, Zhou AD, Li H et al (2006) Expression profile in the cell lines of human TNFAIP1 gene. Yi Chuan 28:918–922. doi:10.1360/yc-006-1330
He H, Tan CK, Downey KM et al (2001) A tumor necrosis factor alpha- and interleukin 6-inducible protein that interacts with the small subunit of DNA polymerase delta and proliferating cell nuclear antigen. Proc Natl Acad Sci USA 98:11979–11984. doi:10.1073/pnas.221452098
Bigger CB, Melnikova IN, Gardner PD (1997) Sp1 and Sp3 regulate expression of the neuronal nicotinic acetylcholine receptor beta4 subunit gene. J Biol Chem 272:25976–25982. doi:10.1074/jbc.272.41.25976
Inoue T, Kamiyama J, Sakai T (1999) Sp1 and NF-Y synergistically mediate the effect of vitamin D(3) in the p27(Kip1) gene promoter that lacks vitamin D response elements. J Biol Chem 274:32309–32317. doi:10.1074/jbc.274.45.32309
Nakamura Y, Kawachi Y, Xu X et al (2007) The combination of ubiquitous transcription factors AP-1 and Sp1 directs keratinocyte-specific and differentiation-specific gene expression in vitro. Exp Dermatol 16:143–150. doi:10.1111/j.1600-0625.2006.00528.x
Richer E, Campion CG, Dabbas B et al (2008) Transcription factors Sp1 and C/EBP regulate NRAMP1 gene expression. FEBS J 275:5074–5089. doi:10.1111/j.1742-4658.2008.06640.x
Haidweger E, Novy M, Rotheneder H (2001) Modulation of Sp1 activity by a cyclin A/CDK complex. J Mol Biol 306:201–212. doi:10.1006/jmbi.2000.4406
Lee JA, Suh DC, Kang JE et al (2005) Transcriptional activity of Sp1 is regulated by molecular interactions between the zinc finger DNA binding domain and the inhibitory domain with corepressors, and this interaction is modulated by MEK. J Biol Chem 280:28061–28071. doi:10.1074/jbc.M414134200
Liu XW, Lu FG, Zhang GS et al (2004) Proteomics to display tissue repair opposing injury response to LPS-induced liver injury. World J Gastroenterol 10:2701–2705
Fiedler U, Reiss Y, Scharpfenecker M et al (2006) Angiopoietin-2 sensitizes endothelial cells to TNF-alpha and has a crucial role in the induction of inflammation. Nat Med 12:235–239. doi:10.1038/nm1351
Danese S, Sans M, de la Motte C et al (2006) Angiogenesis as a novel component of inflammatory bowel disease pathogenesis. Gastroenterology 130:2060–2073. doi:10.1053/j.gastro.2006.03.054
Fiedler U, Augustin HG (2006) Angiopoietins: a link between angiogenesis and inflammation. Trends Immunol 27:552–558. doi:10.1016/j.it.2006.10.004
Ryuto M, Ono M, Izumi H et al (1996) Induction of vascular endothelial growth factor by tumor necrosis factor alpha in human glioma cells. Possible roles of SP-1. J Biol Chem 271:28220–28228. doi:10.1074/jbc.271.45.28220
Ishibashi H, Nakagawa K, Onimaru M et al (2000) Sp1 decoy transfected to carcinoma cells suppresses the expression of vascular endothelial growth factor, transforming growth factor beta1, and tissue factor and also cell growth and invasion activities. Cancer Res 60:6531–6536
Yoshida S, Ono M, Shono T et al (1997) Involvement of interleukin-8, vascular endothelial growth factor, and basic fibroblast growth factor in tumor necrosis factor alpha-dependent angiogenesis. Mol Cell Biol 17:4015–4023
Acknowledgments
This work was supported in part by the 973 project of Ministry of Science and Technique of China (No. 2005CB522505,2006CB943506), the National Natural Science Foundation of China (No. 90608006), the Science and Technology Department of Hunan Province(2007CK3052), and the Hunan Provincial Natural Science Foundation of China (07JJ3075).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Liu, M., Sun, Z., Zhou, A. et al. Functional characterization of the promoter region of human TNFAIP1 gene. Mol Biol Rep 37, 1699–1705 (2010). https://doi.org/10.1007/s11033-009-9588-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11033-009-9588-1