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SATB1 binds an intronic MAR sequence in human PI3kγ in vitro

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Abstract

In our previous study, an intronic MAR sequence in human PI3Kγ gene (PIMAR) was identified using bioinformatics and biochemical methods. We used MatInspector® software to identify potential binding sites for MAR-binding proteins in PIMAR. In this study, a tissue-specific MAR-binding protein (SATB1) was used to characterize the potential binding sites. Southwestern blot analysis indicates that recombinant SATB1 directly binds PIMAR sequence in vitro. Reporter gene assay showed that overexpression of SATB1 downregulates the luciferase reporter linked with reversed PIMAR by approximately threefold in the NIH-3T3 cell line. These results indicate that SATB1 may play antagonistic roles in PI3Kγ transcriptional regulation.

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Acknowledgments

This work was supported by Grant No. 30300066 and No. 30800636 from the China National Nature Science Foundation.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, 280 Chongqing Road (S), 200025, Shanghai, People’s Republic of China

    Rong Cai, Weirong Xu, Bingbing Dai, Xia Cai, Rongting Xu & Jian Lu

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  1. Rong Cai
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  2. Weirong Xu
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  3. Bingbing Dai
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  4. Xia Cai
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  5. Rongting Xu
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  6. Jian Lu
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Corresponding author

Correspondence to Jian Lu.

Additional information

R. Cai and W. Xu contributed equally to this paper.

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Cai, R., Xu, W., Dai, B. et al. SATB1 binds an intronic MAR sequence in human PI3kγ in vitro. Mol Biol Rep 37, 1461–1465 (2010). https://doi.org/10.1007/s11033-009-9538-y

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  • DOI: https://doi.org/10.1007/s11033-009-9538-y

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