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Characterization of a male sterile related gene BcMF15 from Brassica campestris ssp. chinensis

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Abstract

Data from cDNA-AFLP analysis based on the genome-wide transcriptional profiling on the flower buds of the male meiotic cytokinesis (mmc) mutant and its wild-type of Brassica campestris L. ssp. chinensis Makino, syn. B. rapa L. ssp. chinensis, indicated that mutation of the MMC gene resulted in changes in expression of a variety of genes. A transcript-derived fragment specifically accumulated in the wild-type flower buds was isolated, and the corresponding full-length cDNA and DNA was subsequently amplified. Bioinformatical analyses of this gene named BcMF15 (GenBank accession number EF600901) showed that it encoded a protein with 103 amino acids. The BcMF15 had a 88% nucleotide similarity to a lipid transfer protein-like gene. Moreover, sequence prediction indicated that BcMF15 might encode a membrane protein with a signal peptide at the N-terminus. Meanwhile, six domains were predicted in the deduced BcMF15 protein, such as the AAI domain existing in some crucial proteins of pollen development-preferential, signal peptide, transmembrane domain, vWF domain, ZnF_C4 domain, and Tryp_alpha_amyl domain. Spatial and temporal expression patterns analysis by RT-PCR indicated that BcMF15 was exclusively expressed in the fertile line, which indicated this gene is male sterile related. Phylogenetic analysis in Cruciferae revealed that the BcMF15 was relative conservative in evolution. We suppose BcMF15 may be a critical molecule in the transmembrane transportation and signal transduction during microspore development.

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Abbreviations

cDNA-AFLP:

cDNA-amplified fragment length polymorphism

LTP:

Lipid transfer protein

MMC :

Male meiotic cytokinesis

PCR:

Polymerase chain reaction

RACE:

Rapid amplification of cDNA ends

RT-PCR:

Reverse transcriptase polymerase chain reaction

TDF:

Transcript-derived fragment

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Acknowledgements

This work was supported by the Natural Science Foundation of China (No. 30671426) and the Key Sci-technology Project of Zhejiang Province (No. 2005C12019-02).

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Authors and Affiliations

  1. Lab of Cell & Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310029, P.R. China

    Aimei Tian, Jiashu Cao, Li Huang, Xiaolin Yu & Wanzhi Ye

  2. College of Chemistry and Life Science, Three Gorges University, Yichang, 443003, P.R. China

    Aimei Tian

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  1. Aimei Tian
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  2. Jiashu Cao
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  4. Xiaolin Yu
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Correspondence to Jiashu Cao.

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Tian, A., Cao, J., Huang, L. et al. Characterization of a male sterile related gene BcMF15 from Brassica campestris ssp. chinensis . Mol Biol Rep 36, 307–314 (2009). https://doi.org/10.1007/s11033-007-9180-5

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  • DOI: https://doi.org/10.1007/s11033-007-9180-5

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