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Mitochondrial atpA gene is altered in a new orf220-type cytoplasmic male-sterile line of stem mustard (Brassica juncea)

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Abstract

The purpose of this research is to identify the probable mitochondrial factor associated with cytoplasmic male sterility (cms) by comparative analysis of cms and its isogenic maintainer lines in stem mustards. Dramatic variations in the morphology of floral organs were observed in cms stem mustard. Mitochondrial atpA gene was shown to be altered in cms compared with that in its maintainer line, of which mitochondrial atpA gene from its maintainer line was sequenced to encode 507 amino acids. It was indicative of high homology with mitochondrial atpA genes from other species, even as high as 94% in similarity with Oryza sativa in terms of amino acid constituents. However, only 429 amino acids were deduced in cms showing 83% similarity with atpA gene from its maintainer line. Two copies were observed in its maintainer line, but only one was found in cms. Such numerous differences of mitochondrial atpA gene between cms and its maintainer lines may not be the results of evolutionary divergence but the rearrangements of mitochondria. Expression of mitochondrial atpA gene was shown to be down-regulated in cms by using Northern blot. Consequently, mitochondrial ATP synthesis was severely decreased more than one fold in cms stem mustard indicating deficiency in mitochondrial ATP synthesis in this type of cms. Therefore, we deduced that mitochondrial atpA gene altered in cms could be associated with male-sterility in this type of cms.

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Acknowledgements

This work was partially supported by a grant from the National Natural Science Foundation of China (NSFC, 30571270). We thank Dr. Nakazono Mikio (The University of Tokyo) for providing critical comments on the experiment.

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Authors and Affiliations

  1. Laboratory of Genetic Resources & Functional Improvement for Horticultural Plants, Department of Horticulture, Zhejiang University, Hangzhou, 310029, P.R. China

    Jing-Hua Yang, Yan Huai & Ming-Fang Zhang

  2. Laboratory of Horticultural Plant Growth, Development & Biotechnology, Ministry of Agriculture, Hangzhou, 310029, P.R. China

    Jing-Hua Yang, Yan Huai & Ming-Fang Zhang

Authors
  1. Jing-Hua Yang
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  2. Yan Huai
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  3. Ming-Fang Zhang
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Correspondence to Ming-Fang Zhang.

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Jing-Hua Yang and Yan Huai contributed equally to this work.

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Yang, JH., Huai, Y. & Zhang, MF. Mitochondrial atpA gene is altered in a new orf220-type cytoplasmic male-sterile line of stem mustard (Brassica juncea). Mol Biol Rep 36, 273–280 (2009). https://doi.org/10.1007/s11033-007-9176-1

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  • DOI: https://doi.org/10.1007/s11033-007-9176-1

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